Gene expression profile of side population cells in human oral cancer cell line SCC-4

To define the properties of side population (SP) cells of the oral squamous cell　carcinoma cell line SCC-4, we compared their gene expression profile with that of main population (MP) cells. SP cells accounted for approximately 1.5% of the cell population. The gene expression profile in SP cells versus MP cells revealed that 15.8% of genes were up-regulated by more than 10%, and 16.6% were down-regulated by more than 10%. Gene Ontology and KEGG pathway enrichment analyses were carried out on the DAVID online tool for 26,879 distinct probes (p < 0.05). The terms involved in immune/inflammatory responses, cell migration and angiogenesis were detected as up-regulated genes. The terms related to DNA replication, DNA metabolic process, mismatch repair (MMR) and base excretion repair were detected as down-regulated genes. Moreover, mRNAs encoding the ABC transporters ABCG2/BCRP1 and ABCC2/MRP2, which mediate the excretion of anti-cancer drugs such as cisplatin and gefitinib, respectively, were expressed at higher levels. In contrast, the level of mRNA encoding the SLC transporter SLC29A1/ENT1, which mediates the uptake of anti-cancer drugs such as fluorouracil, was decreased. These results indicate that SCC-4 SP cells might be potentially resistant to anti-cancer drugs.

2015

収集根拠 : 博士論文（自動収集）
資料形態 : テキストデータ
コレクション : 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
To define the properties of side population (SP) cells of the oral squamous cell　carcinoma cell line SCC-4, we compared their gene expression profile with that of main population (MP) cells. SP cells accounted for approximately 1.5% of the cell population. The gene expression profile in SP cells versus MP cells revealed that 15.8% of genes were up-regulated by more than 10%, and 16.6% were down-regulated by more than 10%. Gene Ontology and KEGG pathway enrichment analyses were carried out on the DAVID online tool for 26,879 distinct probes (p < 0.05). The terms involved in immune/inflammatory responses, cell migration and angiogenesis were detected as up-regulated genes. The terms related to DNA replication, DNA metabolic process, mismatch repair (MMR) and base excretion repair were detected as down-regulated genes. Moreover, mRNAs encoding the ABC transporters ABCG2/BCRP1 and ABCC2/MRP2, which mediate the excretion of anti-cancer drugs such as cisplatin and gefitinib, respectively, were expressed at higher levels. In contrast, the level of mRNA encoding the SLC transporter SLC29A1/ENT1, which mediates the uptake of anti-cancer drugs such as fluorouracil, was decreased. These results indicate that SCC-4 SP cells might be potentially resistant to anti-cancer drugs.
2015