Mass spectrometric studies on the structures of glycoconjugates

ABSTRACT

CONTENTS

CHAPTER1 Introduction

CHAPTER2 Secondary ion mass spectrometry of sugar nucleotides

2-1 Negative-ion SIMS of sugar nucleotide sodium salts

2-2 MI spectra of〔M-3H+2Na〕⁻,〔M-2H+Na〕⁻ and〔M-H〕⁻ions from negative-ion SIMS

2-3 Linked scan at constant B/E of〔B₁-Na+H〕⁻and〔S₁-Na+H〕⁻ ions

2-4 Positive-ion SIMS of sugar nucleotide sodium salts

2-5 Experimental

2-6 Conclusion

CHAPTER3 Characterization of positional and anomeric isomers of methyl 2-O-and 3-O-sulfo-D-glucopyranosiduronic acids and methyl 2-O-and 3-O-sulfo-D-glucopyranosides by secondary ion mass spectrometry

3-1 Negative-and positive-ion SIMS of methyl O-sulfo-D-glucopyranosiduronic acids and methyl O-sulfo-D-glucopyranosides

3-2 MI spectra of methyl O-sulfo-D-glucopyranosiduronic acids

3-3 MI spectra of methyl O-sulfo-D-glucopyranosides

3-4 Experimental

3-5 Conclusion

CHAPTER4 Tandem mass spectrometry for characterization of unsaturated disaccharides from chondroitin sulfate,dermatan sulfate and hyaluronan

4-1 Positive-and negative-ion FAB mass spectra of non-,mono-,di-and trisulfated unsaturated disaccharides

4-2 MS/MS spectra of the positive-ion FAB of non-,mono-,di-and trisulfated unsaturated disaccharides

4-3 MS/MS spectra of the negative-ion FAB of non-,mono-,di-and trisulfated unsaturated disaccharides

4-4 Experimental

4-5 Conclusion

CHAPTER5 Fast atom bombardment tandem mass spectrometry for characterization of sulfated unsaturated disaccharides from heparin and heparan sulfate

5-1 Negative-ion FAB mass spectra of non-,mono-,di-and trisulfated unsaturated disaccharides

5-2 CID-MS/MS spectra of the negative-ion FAB of GlcNAc-containing unsaturated disaccharides

5-3 CID-MS/MS spectra of the negative-ion FAB of GlcNS-containing unsaturated disaccharides

5-4 CID-MS/MS spectra of the negative-ion FAB of GlcN-containing unsaturated disaccharides

5-5 Positive-ion FAB mass spectra of non-,mono-,di-and trisulfated unsaturated disaccharides

5-6 CID-MS/MS spectra of the positive-ion FAB of GlcNAc-containing unsaturated disaccharides

5-7 CID-MS/MS spectra of the positive-ion FAB of GlcNS-containing unsaturated disaccharides

5-8 CID-MS/MS spectra of the positive-ion FAB of GlcN-containing unsaturated disaccharides

5-9 Experimental

5-10 Conclusion

CHAPTER6 Confirmation of the structures of tri-and tetrasulfated tetrasaccharides obtained from chondroitin sulfate isolated from shark fin

6-1 Tetrasaccharide trisulfate

6-2 Tetrasaccharide tetrasulfate

6-3 Experimental

6-4 Conclusion

CHAPTER7 Electrospray mass spectrometry of pentacosasaccharides of blood group I-activity and related compounds

7-1 Protected linear-chain tri-and decasaccharides

7-2 Fully protected and intact pentaantennary pentacosasaccharide containing nine N-acetyllactosamine groups

7-3 Neutral and acidic glycosphingolipids

7-4 Experimental

7-5 Conclusion

CHAPTER8 Negative-ion electrospray ionization mass spectrometry of glycolipids

8-1 Observation of a novel adduct-ion in negative-ion ESIMS of neutral glycolipids

8-2 Negative-ion ESIMS of neutral and acidic glycolipids

8-3 Experimental

8-4 Conclusion

CHAPTER9 Structural elucidation of underivatized gangliosides by electrospray ionization tandem mass spectrometry

9-1 Negative-ion ESI mass spectra of gangliosides

9-2 Negative-ion ESI CID-MS/MS spectra of gangliosides

9-3 Positive-ion ESI mass spectra of gangliosides

9-4 Positive-ion ESI CID-MS/MS spectra of isomeric gangliosides, GDIa and GDlb

9-5 Experimental

9-6 Conclusion

CHAPTER10 Tandem mass spectrometry for characterization of sulfated Lewis[x] trisaccharides and sulfoglycosphingolipid

10-1 Mono-,di-and trisulfated Le[x] trisacchandes

10-2 Sulfoglycosphingolipid

10-3 Experimental

10-4 Conclusion

CHAPTER11 Conclusion

ACKNOWLEDGMENT

REFERENCES