C2C12筋芽細胞におけるNeuropeptide Y Y1受容体のBMP2による発現制御

DOI 機関リポジトリ (HANDLE) NDLデジタルコレクション

書誌事項

タイトル
C2C12筋芽細胞におけるNeuropeptide Y Y1受容体のBMP2による発現制御
タイトル別名
  • Regulation of neuropeptide Y Y1 receptor expression by bone morphogenetic protein 2 in C2C12 myoblasts
著者
紅林, 奈央子
学位授与大学
北海道大学
取得学位
博士(歯学)
学位授与番号
甲第11268号
学位授与年月日
2014-03-25

説明

The neuropeptide Y(NPY) system is known as one of the major neural signaling pathways. NPY, produced by peripheral tissues including osteoblasts, is known to bind to the Y1 receptor. Recently, osteoblast-specific Y1 receptor knockout mice were developed and were found to have a high bone mass phenotype, indicating a role for the NPY-Y1 receptor axis as a regulator of bone homeostasis. However, regulation of Y1 receptor expression during osteoblastic differentiation remains unexplored. In the present study, we examined the role of bone morphogenetic protein (BMP) 2 signaling in regulating Y1 receptor expression. In C2C12 cells, expression of Y1 receptor mRNA was induced by BMP2. This induction was also observed after co-transfection with Smad1 and Smad4, the intracellular signaling molecules of the BMP2 signaling pathway. In a transfection assay, Smad1/4 up-regulated transcriptional activity through interaction with the Y1 receptor gene promoter. Following transfection of MC3T3-E1 cells with siRNA for the Y1 receptor, the expression of alkaline phosphatase, osteocalcin, Runx2 and osterix were increased. These results show that BMP2 signaling regulates Y1 receptor gene expression, and raises the possibility that NPY acts in osteo blasts via an autocrine mechanism.

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