Solid-State Fermentation with <i>Trichoderma reesei</i> for Cellulase Production
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- D. S. Chahal
- Bacteriology Research Centre, Institut Armand-Frappier, Laval, Quebec, Canada H7V 1B7
抄録
<jats:p> Cellulase yields of 250 to 430 IU/g of cellulose were recorded in a new approach to solid-state fermentation of wheat straw with <jats:italic>Trichoderma reesei</jats:italic> QMY-1. This is an increase of ca. 72% compared with the yields (160 to 250 IU/g of cellulose) in liquid-state fermentation reported in the literature. High cellulase activity (16 to 17 IU/ml) per unit volume of enzyme broth and high yields of cellulases were attributed to the growth of <jats:italic>T. reesei</jats:italic> on a hemicellulose fraction during its first phase and then on a cellulose fraction of wheat straw during its later phase for cellulase production, as well as to the close contact of hyphae with the substrate in solid-state fermentation. The cellulase system obtained by the solid-state fermentation of wheat straw contained cellulases (17.2 IU/ml), β-glucosidase (21.2 IU/ml), and xylanases (540 IU/ml). This cellulase system was capable of hydrolyzing 78 to 90% of delignified wheat straw (10% concentration) in 96 h, without the addition of complementary enzymes, β-glucosidase, and xylanases. </jats:p>
収録刊行物
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- Applied and Environmental Microbiology
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Applied and Environmental Microbiology 49 (1), 205-210, 1985-01
American Society for Microbiology
