Identification of Indicator Proteins Associated with Flooding Injury in Soybean Seedlings Using Label-free Quantitative Proteomics
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- Yohei Nanjo
- NARO Institute of Crop Science, Tsukuba 305-8518, Japan
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- Takuji Nakamura
- NARO Institute of Crop Science, Tsukuba 305-8518, Japan
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- Setsuko Komatsu
- NARO Institute of Crop Science, Tsukuba 305-8518, Japan
書誌事項
- 公開日
- 2013-07-23
- 資源種別
- journal article
- DOI
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- 10.1021/pr4002349
- 公開者
- American Chemical Society (ACS)
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説明
Flooding injury is one of the abiotic constraints on soybean growth. An experimental system established for evaluating flooding injury in soybean seedlings indicated that the degree of injury is dependent on seedling density in floodwater. Dissolved oxygen levels in the floodwater were decreased by the seedlings and correlated with the degree of injury. To understand the molecular mechanism responsible for the injury, proteomic alterations in soybean seedlings that correlated with severity of stress were analyzed using label-free quantitative proteomics. The analysis showed that the abundance of proteins involved in cell wall modification, such as polygalacturonase inhibitor-like and expansin-like B1-like proteins, which may be associated with the defense system, increased dependence on stress at both the protein and mRNA levels in all organs during flooding. The manner of alteration in abundance of these proteins was distinct from those of other responsive proteins. Furthermore, proteins also showing specific changes in abundance in the root tip included protein phosphatase 2A subunit-like proteins, which are possibly involved in flooding-induced root tip cell death. Additionally, decreases in abundance of cell wall synthesis-related proteins, such as cinnamyl-alcohol dehydrogenase and cellulose synthase-interactive protein-like proteins, were identified in hypocotyls of seedlings grown for 3 days after flooding, and these proteins may be associated with suppression of growth after flooding. These flooding injury-associated proteins can be defined as indicator proteins for severity of flooding stress in soybean.
収録刊行物
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- Journal of Proteome Research
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Journal of Proteome Research 12 (11), 4785-4798, 2013-07-23
American Chemical Society (ACS)