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Auxin Sensitivities of All Arabidopsis Aux/IAAs for Degradation in the Presence of Every TIR1/AFB
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Description
Auxin plays a key role in regulation of almost all processes of plant growth and development. Different physiological processes are regulated by different ranges of auxin concentrations; however, the underlying mechanisms creating these differences are largely unknown. The first step of auxin signaling is auxin-dependent interaction of an auxin receptor with transcriptional co-repressors (Aux/IAA), which leads to Aux/IAA degradation. Arabidopsis has six homologous auxin receptors (TIR1 and five AFBs), 29 Aux/IAA proteins and two types of active auxins, IAA and phenylacetic acid (PAA). Therefore, a large number of possible combinations between these three factors may contribute to the creation of complex auxin responses. Using a yeast heterologous reconstitution system, we investigated auxin-dependent degradation of all Arabidopsis Aux/IAAs in combination with every TIR or AFB receptor component. We found that TIR1 and AFB2 were effective in mediating Aux/IAA degradation. We confirmed that the Aux/IAA domain II, which binds TIR1, is essential for degradation. IAA and other natural auxins, 4-chloroindole-3-acetic acid (4-Cl-IAA) and PAA, induced Aux/IAA degradation; and IAA and 4-Cl-IAA had higher activity than PAA. Effective auxin concentrations for Aux/IAA degradation depended on both Aux/IAAs and TIR1 or AFB2 receptors, which is consistent with the Aux/IAA-TIR1/AFB co-receptor concept.
Journal
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- Plant and Cell Physiology
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Plant and Cell Physiology 55 (8), 1450-1459, 2014-05-31
Oxford University Press (OUP)
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Keywords
- Indoleacetic Acids
- Arabidopsis Proteins
- F-Box Proteins
- Molecular Sequence Data
- Arabidopsis
- Nuclear Proteins
- Receptors, Cell Surface
- Plants, Genetically Modified
- DNA-Binding Proteins
- Plant Growth Regulators
- Gene Expression Regulation, Plant
- Genes, Reporter
- Two-Hybrid System Techniques
- Proteolysis
- Amino Acid Sequence
- Sequence Alignment
- Signal Transduction
Details 詳細情報について
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- CRID
- 1360002217652071296
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- ISSN
- 14719053
- 00320781
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- PubMed
- 24880779
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- Article Type
- journal article
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- Data Source
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- Crossref
- KAKEN
- OpenAIRE