VASCULAR-RELATED NAC-DOMAIN6 and VASCULAR-RELATED NAC-DOMAIN7 Effectively Induce Transdifferentiation into Xylem Vessel Elements under Control of an Induction System

DOI PDF PubMed 被引用文献47件 参考文献51件 オープンアクセス
  • Masatoshi Yamaguchi
    RIKEN Plant Science Center, Yokohama, Kanagawa 230–0045, Japan (M.Y., N.G., H.I., M.O., Y.N., N.N., M.K., T.D.); Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630–0192, Japan (M.Y., T.D.); RIKEN Biomass Engineering Program, Yokohama, Kanagawa 230–0045, Japan (M.O., T.D.); Graduate School of Bio-Applications and Systems Engineering, Tokyo University
  • Nadia Goué
    RIKEN Plant Science Center, Yokohama, Kanagawa 230–0045, Japan (M.Y., N.G., H.I., M.O., Y.N., N.N., M.K., T.D.); Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630–0192, Japan (M.Y., T.D.); RIKEN Biomass Engineering Program, Yokohama, Kanagawa 230–0045, Japan (M.O., T.D.); Graduate School of Bio-Applications and Systems Engineering, Tokyo University
  • Hisako Igarashi
    RIKEN Plant Science Center, Yokohama, Kanagawa 230–0045, Japan (M.Y., N.G., H.I., M.O., Y.N., N.N., M.K., T.D.); Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630–0192, Japan (M.Y., T.D.); RIKEN Biomass Engineering Program, Yokohama, Kanagawa 230–0045, Japan (M.O., T.D.); Graduate School of Bio-Applications and Systems Engineering, Tokyo University
  • Misato Ohtani
    RIKEN Plant Science Center, Yokohama, Kanagawa 230–0045, Japan (M.Y., N.G., H.I., M.O., Y.N., N.N., M.K., T.D.); Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630–0192, Japan (M.Y., T.D.); RIKEN Biomass Engineering Program, Yokohama, Kanagawa 230–0045, Japan (M.O., T.D.); Graduate School of Bio-Applications and Systems Engineering, Tokyo University
  • Yoshimi Nakano
    RIKEN Plant Science Center, Yokohama, Kanagawa 230–0045, Japan (M.Y., N.G., H.I., M.O., Y.N., N.N., M.K., T.D.); Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630–0192, Japan (M.Y., T.D.); RIKEN Biomass Engineering Program, Yokohama, Kanagawa 230–0045, Japan (M.O., T.D.); Graduate School of Bio-Applications and Systems Engineering, Tokyo University
  • Jennifer C. Mortimer
    RIKEN Plant Science Center, Yokohama, Kanagawa 230–0045, Japan (M.Y., N.G., H.I., M.O., Y.N., N.N., M.K., T.D.); Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630–0192, Japan (M.Y., T.D.); RIKEN Biomass Engineering Program, Yokohama, Kanagawa 230–0045, Japan (M.O., T.D.); Graduate School of Bio-Applications and Systems Engineering, Tokyo University
  • Nobuyuki Nishikubo
    RIKEN Plant Science Center, Yokohama, Kanagawa 230–0045, Japan (M.Y., N.G., H.I., M.O., Y.N., N.N., M.K., T.D.); Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630–0192, Japan (M.Y., T.D.); RIKEN Biomass Engineering Program, Yokohama, Kanagawa 230–0045, Japan (M.O., T.D.); Graduate School of Bio-Applications and Systems Engineering, Tokyo University
  • Minoru Kubo
    RIKEN Plant Science Center, Yokohama, Kanagawa 230–0045, Japan (M.Y., N.G., H.I., M.O., Y.N., N.N., M.K., T.D.); Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630–0192, Japan (M.Y., T.D.); RIKEN Biomass Engineering Program, Yokohama, Kanagawa 230–0045, Japan (M.O., T.D.); Graduate School of Bio-Applications and Systems Engineering, Tokyo University
  • Yoshihiro Katayama
    RIKEN Plant Science Center, Yokohama, Kanagawa 230–0045, Japan (M.Y., N.G., H.I., M.O., Y.N., N.N., M.K., T.D.); Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630–0192, Japan (M.Y., T.D.); RIKEN Biomass Engineering Program, Yokohama, Kanagawa 230–0045, Japan (M.O., T.D.); Graduate School of Bio-Applications and Systems Engineering, Tokyo University
  • Koichi Kakegawa
    RIKEN Plant Science Center, Yokohama, Kanagawa 230–0045, Japan (M.Y., N.G., H.I., M.O., Y.N., N.N., M.K., T.D.); Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630–0192, Japan (M.Y., T.D.); RIKEN Biomass Engineering Program, Yokohama, Kanagawa 230–0045, Japan (M.O., T.D.); Graduate School of Bio-Applications and Systems Engineering, Tokyo University
  • Paul Dupree
    RIKEN Plant Science Center, Yokohama, Kanagawa 230–0045, Japan (M.Y., N.G., H.I., M.O., Y.N., N.N., M.K., T.D.); Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630–0192, Japan (M.Y., T.D.); RIKEN Biomass Engineering Program, Yokohama, Kanagawa 230–0045, Japan (M.O., T.D.); Graduate School of Bio-Applications and Systems Engineering, Tokyo University
  • Taku Demura
    RIKEN Plant Science Center, Yokohama, Kanagawa 230–0045, Japan (M.Y., N.G., H.I., M.O., Y.N., N.N., M.K., T.D.); Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630–0192, Japan (M.Y., T.D.); RIKEN Biomass Engineering Program, Yokohama, Kanagawa 230–0045, Japan (M.O., T.D.); Graduate School of Bio-Applications and Systems Engineering, Tokyo University

書誌事項

公開日
2010-05-20
資源種別
journal article
権利情報
  • https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model
DOI
  • 10.1104/pp.110.154013
公開者
Oxford University Press (OUP)

説明

<jats:title>Abstract</jats:title> <jats:p>We previously showed that the VASCULAR-RELATED NAC-DOMAIN6 (VND6) and VND7 genes, which encode NAM/ATAF/CUC domain protein transcription factors, act as key regulators of xylem vessel differentiation. Here, we report a glucocorticoid-mediated posttranslational induction system of VND6 and VND7. In this system, VND6 or VND7 is expressed as a fused protein with the activation domain of the herpes virus VP16 protein and hormone-binding domain of the animal glucocorticoid receptor, and the protein's activity is induced by treatment with dexamethasone (DEX), a glucocorticoid derivative. Upon DEX treatment, transgenic Arabidopsis (Arabidopsis thaliana) plants carrying the chimeric gene exhibited transdifferentiation of various types of cells into xylem vessel elements, and the plants died. Many genes involved in xylem vessel differentiation, such as secondary wall biosynthesis and programmed cell death, were up-regulated in these plants after DEX treatment. Chemical analysis showed that xylan, a major hemicellulose component of the dicot secondary cell wall, was increased in the transgenic plants after DEX treatment. This induction system worked in poplar (Populus tremula × tremuloides) trees and in suspension cultures of cells from Arabidopsis and tobacco (Nicotiana tabacum); more than 90% of the tobacco BY-2 cells expressing VND7-VP16-GR transdifferentiated into xylem vessel elements after DEX treatment. These data demonstrate that the induction systems controlling VND6 and VND7 activities can be used as powerful tools for understanding xylem cell differentiation.</jats:p>

収録刊行物

  • Plant Physiology

    Plant Physiology 153 (3), 906-914, 2010-05-20

    Oxford University Press (OUP)

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