Production of xylitol from D-xylulose by Mycobacterium smegmatis

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Abstract Mycobacterium smegmatis transformed D -xylulose to xylitol in washed cell reactions under aerobic and anaerobic conditions. The yield of xylitol reached about 70% in anaerobic conditions (in N 2 ) by cells grown on media containing xylitol or D -mannitol. Cells immobilized with Ca-alginate had almost the same activity of xylitol production as washed cells. Xylitol was produced from D -xylose using commercial immobilized D -xylose isomerase from Bacillus coagulans and immobilized cells of M. smegmatis . From 10 g of D -xylose, 4 g of xylitol was produced and 5 g of D -xylose remained in the reaction mixture; no D -xylulose was detected.

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