Deadenylation by the <scp>CCR</scp>4‐<scp>NOT</scp> complex contributes to the turnover of <i>hairy</i>‐related <scp>mRNA</scp>s in the zebrafish segmentation clock

DOI Web Site Web Site Web Site Web Site ほか1件をすべて表示 一部だけ表示 被引用文献1件 参考文献41件
  • Yuuri Fujino
    Division of Life Science Graduate School of Science and Engineering Saitama University Japan
  • Kazuya Yamada
    Division of Life Science Graduate School of Science and Engineering Saitama University Japan
  • Chihiro Sugaya
    Division of Life Science Graduate School of Science and Engineering Saitama University Japan
  • Yuko Ooka
    Division of Life Science Graduate School of Science and Engineering Saitama University Japan
  • Hiroki Ovara
    Division of Life Science Graduate School of Science and Engineering Saitama University Japan
  • Hiroyuki Ban
    Division of Life Science Graduate School of Science and Engineering Saitama University Japan
  • Kagari Akama
    Division of Life Science Graduate School of Science and Engineering Saitama University Japan
  • Shiori Otosaka
    Division of Life Science Graduate School of Science and Engineering Saitama University Japan
  • Hirofumi Kinoshita
    Division of Life Science Graduate School of Science and Engineering Saitama University Japan
  • Kyo Yamasu
    Division of Life Science Graduate School of Science and Engineering Saitama University Japan
  • Yuichiro Mishima
    Department of Molecular Biosciences Faculty of Life Sciences Kyoto Sangyo University Japan
  • Akinori Kawamura
    Division of Life Science Graduate School of Science and Engineering Saitama University Japan

抄録

<jats:sec><jats:label /><jats:p>In the zebrafish segmentation clock, <jats:italic>hairy/enhancer of split</jats:italic>‐related genes <jats:italic>her1</jats:italic>,<jats:italic> her7</jats:italic>, and <jats:italic>hes6</jats:italic> encodes components of core oscillators. Since the expression of cyclic genes proceeds rapidly in the presomitic mesoderm (<jats:styled-content style="fixed-case">PSM</jats:styled-content>), these <jats:italic>hairy</jats:italic>‐related <jats:styled-content style="fixed-case">mRNA</jats:styled-content>s are subject to strict post‐transcriptional regulation. In this study, we demonstrate that inhibition of the <jats:styled-content style="fixed-case">CCR</jats:styled-content>4‐<jats:styled-content style="fixed-case">NOT</jats:styled-content> deadenylase complex lengthens poly(A) tails of <jats:italic>hairy</jats:italic>‐related <jats:styled-content style="fixed-case">mRNA</jats:styled-content>s and increases the amount of these <jats:styled-content style="fixed-case">mRNA</jats:styled-content>s, which is accompanied by defective somite segmentation. In transgenic embryos, we show that <jats:italic><jats:styled-content style="fixed-case">EGFP</jats:styled-content></jats:italic> <jats:styled-content style="fixed-case">mRNA</jats:styled-content>s with 3′<jats:styled-content style="fixed-case">UTR</jats:styled-content>s of <jats:italic>hairy</jats:italic>‐related genes exhibit turnover similar to endogenous <jats:styled-content style="fixed-case">mRNA</jats:styled-content>s. Our results suggest that turnover rates of <jats:italic>her1, her7,</jats:italic> and <jats:italic>hes6 </jats:italic><jats:styled-content style="fixed-case">mRNA</jats:styled-content>s are differently regulated by the <jats:styled-content style="fixed-case">CCR</jats:styled-content>4‐<jats:styled-content style="fixed-case">NOT</jats:styled-content> deadenylase complex possibly through their 3′<jats:styled-content style="fixed-case">UTR</jats:styled-content>s in the zebrafish PSM.</jats:p></jats:sec>

収録刊行物

  • FEBS Letters

    FEBS Letters 592 (20), 3388-3398, 2018-10

    Wiley

被引用文献 (1)*注記

もっと見る

参考文献 (41)*注記

もっと見る

関連プロジェクト

もっと見る

問題の指摘

ページトップへ