Unveiling the Biosynthetic Pathway of the Ribosomally Synthesized and Post‐translationally Modified Peptide Ustiloxin B in Filamentous Fungi

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  • Ying Ye
    Division of Chemistry, Graduate School of Science Hokkaido University Sapporo 060-0810 Japan
  • Atsushi Minami
    Division of Chemistry, Graduate School of Science Hokkaido University Sapporo 060-0810 Japan
  • Yuya Igarashi
    Division of Chemistry, Graduate School of Science Hokkaido University Sapporo 060-0810 Japan
  • Miho Izumikawa
    Biomedicinal Information Research Center (BIRC) Japan Biological Informatics Consortium (JBIC), Koto-ku Tokyo 818 Japan
  • Myco Umemura
    Bioproduction Research Institute National Institute of Advanced Industrial Science and Technology (AIST) Sapporo 062-8517 Japan
  • Nozomi Nagano
    National Institute of Advanced Industrial Science and Technology (AIST) Koto-ku, Tokyo 135-0064 Japan
  • Masayuki Machida
    Bioproduction Research Institute National Institute of Advanced Industrial Science and Technology (AIST) Sapporo 062-8517 Japan
  • Teppei Kawahara
    Biomedicinal Information Research Center (BIRC) Japan Biological Informatics Consortium (JBIC), Koto-ku Tokyo 818 Japan
  • Kazuo Shin‐ya
    National Institute of Advanced Industrial Science and Technology (AIST) Koto-ku, Tokyo 135-0064 Japan
  • Katsuya Gomi
    Graduate School of Agricultural Science Tohoku University Sendai 981-8555 Japan
  • Hideaki Oikawa
    Division of Chemistry, Graduate School of Science Hokkaido University Sapporo 060-0810 Japan

Abstract

<jats:title>Abstract</jats:title><jats:p>The biosynthetic machinery of the first fungal ribosomally synthesized and post‐translationally modified peptide (RiPP) ustiloxin B was elucidated through a series of gene inactivation and heterologous expression studies. The results confirmed an essential requirement for novel oxidases possessing the DUF3328 motif for macrocyclization, and highly unique side‐chain modifications by three oxidases (UstCF1F2) and a pyridoxal 5′‐phosphate (PLP)‐dependent enzyme (UstD). These findings provide new insight into the expression of the RiPP gene clusters found in various fungi.</jats:p>

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