Involvement of Transient Receptor Potential Cation Channel Vanilloid 1 (TRPV1) in Myoblast Fusion
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- Mitsutoshi Kurosaka
- Department of Physiology St. Marianna University School of Medicine Kawasaki Kanagawa Japan
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- Yuji Ogura
- Department of Physiology St. Marianna University School of Medicine Kawasaki Kanagawa Japan
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- Toshiya Funabashi
- Department of Physiology St. Marianna University School of Medicine Kawasaki Kanagawa Japan
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- Tatsuo Akema
- Department of Physiology St. Marianna University School of Medicine Kawasaki Kanagawa Japan
書誌事項
- 公開日
- 2016-03-08
- 資源種別
- journal article
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1002/jcp.25345
- 公開者
- Wiley
この論文をさがす
説明
<jats:sec><jats:label/><jats:p>The mechanisms that underlie the complex process of muscle regeneration after injury remain unknown. Transient receptor potential cation channel vanilloid 1 (TRPV1) is expressed in several cell types, including skeletal muscle, and is activated by high temperature and by certain molecules secreted during tissue inflammation. Severe inflammation and local temperature perturbations are induced during muscle regeneration, which suggests that TRPV1 might be activated and involved in the process. The aim of this study, was to clarify the role of TRPV1 in the myogenic potential of satellite cells responsible for muscle regeneration. We found that mRNA and protein levels of TRPV1 increased during regeneration after cardiotoxin (CTX)‐induced muscle injury in mice. Using isolated mouse satellite cells (i.e., myoblasts), we observed that activation of TRPV1 by its agonist capsaicin (CAP) augmented myogenin protein levels. Whereas CAP did not alter myoblast proliferation, it facilitated myoblast fusion (evaluated using myonucleii number per myotube and fusion index). In contrast, suppression of TRPV1 by siRNA impaired myoblast fusion. Using mice, we also demonstrated that intramuscular injection of CAP facilitated muscle repair after CTX‐induced muscle injury. Moreover, we showed that these roles of TRPV1 might be mediated by interleukin‐4 and calcium signaling during myoblast fusion. Collectively, these results suggest that TRPV1 underlies normal myogenesis through promotion of myoblast fusion. J. Cell. Physiol. 231: 2275–2285, 2016. © 2016 Wiley Periodicals, Inc.</jats:p></jats:sec>
収録刊行物
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- Journal of Cellular Physiology
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Journal of Cellular Physiology 231 (10), 2275-2285, 2016-03-08
Wiley