Therapeutic potential of hyaluronan oligosaccharides for bone metastasis of breast cancer
書誌事項
- 公開日
- 2011-09-12
- 資源種別
- journal article
- 権利情報
-
- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
-
- 10.1002/jor.21557
- 公開者
- Wiley
この論文をさがす
説明
<jats:title>Abstract</jats:title><jats:p>Hyaluronan (HA) oligosaccharides were reported to have suppressive effects on various malignant tumors via disruption of receptor HA interactions. However, no studies have focused on the effects of HA oligosaccharides on bone metastasis of breast cancer. In this study, we clarified the effective size of HA oligosaccharides required to inhibit cell growth in the highly invasive breast cancer cell line, MDA‐MB‐231 cells. Based on the results of cell growth assay, we subsequently analyzed the effects of HA tetrasaccharides, HA decasaccharides, and high molecular weight HA on the other breast cancer cell behaviors in vitro and breast cancer bone metastasis in vivo. HA decasaccharides significantly inhibited cell growth, motility, and invasion, whereas tetrasaccharides did not. HAS2 mRNA expression was altered after the treatment with both tetrasaccharides and decasaccharides. Phosphorylation of Akt was suppressed after 1 h treatment with HA decasaccharides, and the effect was partially reversed by anti‐CD44 monoclonal antibody. In vivo, local application of HA decasaccharides inhibited the expansion of osteolytic lesions in tibia on soft X‐rays using mouse bone metastasis model of breast cancer. Histological analysis revealed HA accumulation in bone metastatic lesions was perturbed by decasaccharides. These results suggest that HA oligosaccharides suppressed progression of bone metastasis in breast cancer via interruption of endogenous HA–CD44 interaction, and as such, can be a novel therapeutic candidate to limit bone metastasis of breast cancer. © 2011 Orthopaedic Research Society. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 30:662–672, 2012</jats:p>
収録刊行物
-
- Journal of Orthopaedic Research
-
Journal of Orthopaedic Research 30 (4), 662-672, 2011-09-12
Wiley
- Tweet
キーワード
- Oligosaccharides
- Bone Neoplasms
- Breast Neoplasms
- Mice
- Cell Movement
- Cell Line, Tumor
- Animals
- Humans
- Neoplasm Invasiveness
- Glucuronosyltransferase
- Hyaluronic Acid
- Phosphorylation
- Tibia
- Viscosupplements
- Xenograft Model Antitumor Assays
- Gene Expression Regulation, Neoplastic
- Disease Models, Animal
- Hyaluronan Receptors
- NIH 3T3 Cells
- Female
- Hyaluronan Synthases
- Proto-Oncogene Proteins c-akt
- Cell Division
詳細情報 詳細情報について
-
- CRID
- 1360004230193036928
-
- ISSN
- 1554527X
- 07360266
-
- PubMed
- 21913222
-
- 資料種別
- journal article
-
- データソース種別
-
- Crossref
- KAKEN
- OpenAIRE
