A Fluorescent Imaging Probe Based on a Macrocyclic Scaffold That Binds to Cellular EpCAM
書誌事項
- 公開日
- 2015-11-09
- 資源種別
- journal article
- 権利情報
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- http://www.springer.com/tdm
- DOI
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- 10.1007/s00239-015-9710-z
- 公開者
- Springer Science and Business Media LLC
この論文をさがす
説明
Here, we report a fluorescent probe based on a macrocyclic peptide scaffold that specifically stains EpCAM-expressing MCF7 cells. The 14-mer macrocyclic peptide binding to the extracellular domain of EpCAM with a dissociation constant in the low nM range (1.7 nM) was discovered using the random non-standard peptide-integrated discovery system. Notably, this probe containing a fluorescence tag is less than 3000 Da in total and able to visualize nearly every live cell under high cell-density conditions, which was not achieved by the conventional mAb staining method. This suggests that the molecular probe based on the compact macrocyclic scaffold has great potentials as an imaging tool for the EpCAM biomarker as well as a delivery vehicle for drug conjugates.
収録刊行物
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- Journal of Molecular Evolution
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Journal of Molecular Evolution 81 (5-6), 210-217, 2015-11-09
Springer Science and Business Media LLC