GRK-6 mediates FSH action synergistically enhanced by estrogen and the oocyte in rat granulosa cells
書誌事項
- 公開日
- 2013-05
- 資源種別
- journal article
- 権利情報
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- https://www.elsevier.com/tdm/userlicense/1.0/
- DOI
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- 10.1016/j.bbrc.2013.04.002
- 公開者
- Elsevier BV
この論文をさがす
説明
Estrogen is known to play a pivotal role in granulosa cell responses to follicle-stimulating hormone (FSH) that is critical for the establishment of dominant follicles and subsequent ovulation in mammals. Thus, elucidating the cellular and molecular mechanisms that regulate FSH activity is important to understand female fertility. We previously discovered that the oocyte is required for estrogen to exert its positive effects on FSH activity in rat granulosa cells. This finding supports the new concept that estrogen action in granulosa cells is mediated by the oocyte. In the current study, we explored the underlying mechanism. In the presence of oocytes, estrogens enhanced FSH-induced increases in aromatase, steroidogenic acute regulatory protein and FSH receptor mRNA expression as well as cAMP production. However, as forskolin did not mimic FSH activity this indicated that coexistence of estrogen/oocytes increases FSH activity at a site upstream of adenylate cyclase in granulosa cells. We therefore sought a possible involvement of the autoregulatory molecules for FSH receptor, G protein-coupled receptor kinases (GRKs) and ß-arrestins in enhancing FSH activity in response to the estrogen/oocyte co-treatment in granulosa cells. Among the seven known GRK and two ß-arrestin molecules, we found that estrogens with oocytes suppressed FSH-induced GRK-6 mRNA expression. Consistent with this finding, transfecting granulosa cells with small interfering RNA of GRK-6 significantly increased FSH induction of aromatase mRNA, suggesting that endogenous GRK-6 plays an inhibitory role in FSH-induced aromatase mRNA expression. Consequently, these findings strongly suggest that GRK-6 is involved in the mechanism by which estrogen and oocytes synergistically augment FSH activity in granulosa cells.
収録刊行物
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- Biochemical and Biophysical Research Communications
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Biochemical and Biophysical Research Communications 434 (2), 401-406, 2013-05
Elsevier BV
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キーワード
- Granulosa Cells
- Primary Cell Culture
- Drug Synergism
- Estrogens
- G-Protein-Coupled Receptor Kinases
- Transfection
- Coculture Techniques
- Rats
- Rats, Sprague-Dawley
- Aromatase
- Cyclic AMP
- Oocytes
- Animals
- Receptors, FSH
- Female
- RNA, Messenger
- Follicle Stimulating Hormone
- RNA, Small Interfering
- Diethylstilbestrol
- Cells, Cultured
詳細情報 詳細情報について
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- CRID
- 1360004232000761216
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- ISSN
- 0006291X
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- PubMed
- 23583200
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- 資料種別
- journal article
-
- データソース種別
-
- Crossref
- KAKEN
- OpenAIRE
