Protein labeling with fluorogenic probes for no-wash live-cell imaging of proteins
説明
Protein labeling by using a protein tag and its specific fluorescent probe is increasingly becoming a useful technique for the real-time imaging of proteins in living cells. Recently, fluorogenic probes for protein labeling were developed. When using these probes, a washing step is not required for the removal of free probes from the cells, thus, allowing rapid detection of proteins in living cells with high signal-to-noise ratio. Various chemical principles have been applied in the designing of probes to include a turn-on fluorescence switch that is activated by the protein labeling reaction. In this review, we describe about the design strategy of the probes and the advances in fluorogenic protein labeling systems.
収録刊行物
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- Current Opinion in Chemical Biology
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Current Opinion in Chemical Biology 17 (4), 644-650, 2013-08
Elsevier BV
