Histone Variant H2A.L.2 Guides Transition Protein-Dependent Protamine Assembly in Male Germ Cells
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説明
Histone replacement by transition proteins (TPs) and protamines (Prms) constitutes an essential step for the successful production of functional male gametes, yet nothing is known on the underlying functional interplay between histones, TPs, and Prms. Here, by studying spermatogenesis in the absence of a spermatid-specific histone variant, H2A.L.2, we discover a fundamental mechanism involved in the transformation of nucleosomes into nucleoprotamines. H2A.L.2 is synthesized at the same time as TPs and enables their loading onto the nucleosomes. TPs do not displace histones but rather drive the recruitment and processing of Prms, which are themselves responsible for histone eviction. Altogether, the incorporation of H2A.L.2 initiates and orchestrates a series of successive transitional states that ultimately shift to the fully compacted genome of the mature spermatozoa. Hence, the current view of histone-to-nucleoprotamine transition should be revisited and include an additional step with H2A.L.2 assembly prior to the action of TPs and Prms.
収録刊行物
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- Molecular Cell
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Molecular Cell 66 (1), 89-101.e8, 2017-04
Elsevier BV
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キーワード
- Male
- Mice, 129 Strain
- Chromosomal Proteins, Non-Histone
- [SDV]Life Sciences [q-bio]
- Transfection
- Histones
- Chlorocebus aethiops
- Databases, Genetic
- Animals
- Genetic Predisposition to Disease
- Protamines
- Spermatogenesis
- Infertility, Male
- Mice, Knockout
- Genome
- Computational Biology
- Gene Expression Regulation, Developmental
- Chromatin Assembly and Disassembly
- Spermatozoa
- Chromatin
- Nucleosomes
- Fertility
- Phenotype
- COS Cells
詳細情報 詳細情報について
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- CRID
- 1360004232375953664
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- ISSN
- 10972765
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- PubMed
- 28366643
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- 資料種別
- journal article
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- データソース種別
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- Crossref
- KAKEN
- OpenAIRE