Huddling facilitates expression of daily torpor in the large Japanese field mouse Apodemus speciosus
書誌事項
- 公開日
- 2014-06
- 資源種別
- journal article
- 権利情報
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- https://www.elsevier.com/tdm/userlicense/1.0/
- DOI
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- 10.1016/j.physbeh.2014.04.051
- 10.1007/s13105-014-0353-0
- 公開者
- Elsevier BV
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説明
The influence of human serum albumin (HSA) on the bile acid-mediated inhibition of liver microsomal type 1 11β-hydroxysteroid dehydrogenase (11β-HSD1) was studied in vitro. A rat liver microsomal fraction was prepared, and the 11β-HSD1 enzyme activity in the presence of various concentrations of bile acids and HSA was determined using hydrocortisone as the substrate. The products of the reaction were extracted and analyzed using high-performance liquid chromatography. The magnitude of the inhibition decreased with the addition of HSA in a dose-dependent manner. Four percent human albumin decreased the inhibitory effects of 100 μM chenodeoxycholic acid and lithocholic acid from 89.9 ± 5.6 to 54.5 ± 6.1% and from 83.8 ± 4.8 to 20.8 ± 4.2%, respectively. In contrast, ursodeoxycholic acid and deoxycholic acid showed no inhibitory effect on the enzyme activity in the presence of 4% human serum albumin, and the addition of 1% γ-globulin to the assay mixture in the presence of bile acids did not affect the enzyme activity. Our in vitro study showed that the addition of HSA ameliorated the inhibition of 11β-HSD1 and that the magnitude of the change is dependent on the species of bile acid, presumably based on the numbers of hydroxyl groups. These results suggest that HSA seems to protect the bile acid-mediated inhibition of 11β-HSD1 in the healthy subject. On the other hand, in the patients with obstructive biliary diseases, not only elevated serum bile acid but also the accompanying hypoalbuminemia is important to evaluate the pathophysiology of the bile acid-mediated inhibition of 11β-HSD1 of the disease.
収録刊行物
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- Physiology & Behavior
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Physiology & Behavior 133 22-29, 2014-06
Elsevier BV
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詳細情報 詳細情報について
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- CRID
- 1360004232431678080
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- ISSN
- 18778755
- 00319384
- 11387548
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- https://api.elsevier.com/content/article/PII:S003193841400242X?httpAccept=text/xml
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- http://link.springer.com/content/pdf/10.1007/s13105-014-0353-0.pdf
- http://link.springer.com/article/10.1007/s13105-014-0353-0/fulltext.html
- http://link.springer.com/content/pdf/10.1007/s13105-014-0353-0
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- 資料種別
- journal article
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