Surface-Engineered Biocatalytic Composite Membranes for Reduced Protein Fouling and Self-Cleaning
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- Anbharasi Vanangamudi
- Institute for Frontier Materials, Deakin University, Waurn Ponds, Victoria 3216, Australia
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- Daisuke Saeki
- Department of Chemical Science and Engineering, Kobe University, 1-1 Rokkodai-cho, Nada, Kobe, Hyogo 657-8501, Japan
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- Ludovic F. Dumée
- Institute for Frontier Materials, Deakin University, Waurn Ponds, Victoria 3216, Australia
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- Hideto Matsuyama
- Department of Chemical Science and Engineering, Kobe University, 1-1 Rokkodai-cho, Nada, Kobe, Hyogo 657-8501, Japan
書誌事項
- 公開日
- 2018-07-26
- 資源種別
- journal article
- DOI
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- 10.1021/acsami.8b07945
- 公開者
- American Chemical Society (ACS)
この論文をさがす
説明
A new biocatalytic nanofibrous composite ultrafiltration membrane was developed to reduce protein fouling interactions and self-clean the membrane surface. The dual-layer poly(vinylidenefluoride)/nylon-6,6/chitosan composite membrane contains a hydrophobic poly(vinylidenefluoride) cast support layer and a hydrophilic functional nylon-6,6/chitosan nanofibrous surface layer where enzymes were chemically attached. The intrinsic surface chemistry and high surface area of the nanofibers allowed optimal and stable immobilization of trypsin (TR) and α-chymotrypsin enzymes via direct covalent binding. The enzyme immobilization was confirmed by X-ray photoelectron spectroscopy and visualized by confocal microscopy analysis. The prepared biocatalytic composite membranes were nanoporous with superior permeability offering stable protein antiadhesion and self-cleaning properties owing to the repulsive mechanism and digestion of proteins into peptides and amino acids, which was quantified by the gel electrophoresis technique. The TR-immobilized composite membranes exhibited 2.7-fold higher permeance and lower surface protein contamination with 3-fold greater permeance recovery, when compared to the pristine membrane after two ultrafiltration cycles with the model feed solution containing bovine serum albumin/NaCl/CaCl2. The biocatalytic membranes retained about 50% of the enzyme activity after six reuse cycles but were regenerated to 100% activity after enzyme reloading, leading to a simple and cost-effective water remediation operation. Such surface- and pore-engineered membranes with self-cleaning properties offer a viable solution for severe surface protein contamination in food and water applications.
収録刊行物
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- ACS Applied Materials & Interfaces
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ACS Applied Materials & Interfaces 10 (32), 27477-27487, 2018-07-26
American Chemical Society (ACS)
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キーワード
- College of Health and Biomedicine
- 0904 Chemical Engineering
- Ultrafiltration
- Membranes, Artificial
- Enzymes, Immobilized
- protein fouling
- Permeability
- trypsin
- College of Science and Engineering
- Biocatalysis
- ultrafiltration membrane
- self-cleaning membranes
- Institute for Sustainability and Innovation (ISI)
- Hydrophobic and Hydrophilic Interactions
- biocatalytic membranes
詳細情報 詳細情報について
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- CRID
- 1360004233146471424
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- ISSN
- 19448252
- 19448244
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- PubMed
- 30048587
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- 資料種別
- journal article
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- データソース種別
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- Crossref
- KAKEN
- OpenAIRE