Phytomonitoring and Phytoremediation of Agrochemicals and Related Compounds Based on Recombinant Cytochrome P450s and Aryl Hydrocarbon Receptors (AhRs)
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- Sayuri Shimazu
- Research Center for Green Science, Fukuyama University, Fukuyama, Hiroshima, 729-0292 Japan
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- Hideyuki Inui
- Research Center for Environmental Genomics, Kobe University, Kobe, Hyogo, 657-8501 Japan
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- Hideo Ohkawa
- Research Center for Green Science, Fukuyama University, Fukuyama, Hiroshima, 729-0292 Japan
書誌事項
- 公開日
- 2010-09-30
- 資源種別
- journal article
- DOI
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- 10.1021/jf102561d
- 公開者
- American Chemical Society (ACS)
この論文をさがす
説明
Molecular mechanisms of metabolism and modes of actions of agrochemicals and related compounds are important for understanding selective toxicity, biodegradability, and monitoring of biological effects on nontarget organisms. It is well-known that in mammals, cytochrome P450 (P450 or CYP) monooxygenases metabolize lipophilic foreign compounds. These P450 species are inducible, and both CYP1A1 and CYP1A2 are induced by aryl hydrocarbon receptor (AhR) combined with a ligand. Gene engineering of P450 and NADPH cytochrome P450 oxidoreductase (P450 reductase) was established for bioconversion. Also, gene modification of AhRs was developed for recombinant AhR-mediated β-glucronidase (GUS) reporter assay of AhR ligands. Recombinant P450 genes were transformed into plants for phytoremediation, and recombinant AhR-mediated GUS reporter gene expression systems were each transformed into plants for phytomonitoring. Transgenic rice plants carrying CYP2B6 metabolized the herbicide metolachlor and remarkably reduced the residues in the plants and soils under paddy field conditions. Transgenic Arabidopsis plants carrying recombinant guinea pig (g) AhR-mediated GUS reporter genes detected PCB126 at the level of 10 ng/g soils in the presence of biosurfactants MEL-B. Both phytomonitoring and phytoremediation plants were each evaluated from the standpoint of practical uses.
収録刊行物
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- Journal of Agricultural and Food Chemistry
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Journal of Agricultural and Food Chemistry 59 (7), 2870-2875, 2010-09-30
American Chemical Society (ACS)