Structural basis for the recognition–evasion arms race between <i>Tomato mosaic virus</i> and the resistance gene <i>Tm-1</i>

  • Kazuhiro Ishibashi
    Plant-Microbe Interactions Research Unit and
  • Yuichiro Kezuka
    Department of Structural Biology, School of Pharmacy, Iwate Medical University, Yahaba, Iwate 020-0023, Japan; and
  • Chihoko Kobayashi
    Biomolecular Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan;
  • Masahiko Kato
    Biomolecular Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan;
  • Tsuyoshi Inoue
    Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan
  • Takamasa Nonaka
    Department of Structural Biology, School of Pharmacy, Iwate Medical University, Yahaba, Iwate 020-0023, Japan; and
  • Masayuki Ishikawa
    Plant-Microbe Interactions Research Unit and
  • Hiroyoshi Matsumura
    Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan
  • Etsuko Katoh
    Biomolecular Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan;

書誌事項

公開日
2014-08-04
資源種別
journal article
DOI
  • 10.1073/pnas.1407888111
公開者
Proceedings of the National Academy of Sciences

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説明

<jats:title>Significance</jats:title> <jats:p>The Red Queen hypothesis proposes that host defense genes evolve to counter the adverse effects of rapidly evolving invasive viruses. Although 3D structures of host–viral protein complexes have provided great insights into the molecular conflicts between them, a single structure represents only an evolutionary snapshot. Here we present the atomic details of the step-by-step arms race between tomato mosaic virus replication protein and the host inhibitor protein Tm-1, in which host recognition of a viral molecule, viral adaptive evasion of the recognition, host counteradaptation, and viral counter-counteradaptation are depicted by determination of the complex structures of Tm-1 variants and the viral protein and by biochemical analyses and molecular dynamics simulations of the interactions between these proteins.</jats:p>

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