Influenza A virus nucleoprotein is acetylated by histone acetyltransferases PCAF and GCN5
書誌事項
- 公開日
- 2018-05
- 資源種別
- journal article
- 権利情報
-
- https://www.elsevier.com/tdm/userlicense/1.0/
- http://creativecommons.org/licenses/by/4.0/
- DOI
-
- 10.1074/jbc.ra117.001683
- 公開者
- Elsevier BV
この論文をさがす
説明
Histone acetylation plays crucial roles in transcriptional regulation and chromatin organization. Viral RNA of the influenza virus interacts with its nucleoprotein (NP), whose function corresponds to that of eukaryotic histones. NP regulates viral replication and has been shown to undergo acetylation by the cAMP-response element (CRE)-binding protein (CBP) from the host. However, whether NP is the target of other host acetyltransferases is unknown. Here, we show that influenza virus NP undergoes acetylation by the two host acetyltransferases GCN5 and P300/CBP-associated factor (PCAF) and that this modification affects viral polymerase activities. Western blot analysis with anti-acetyl-lysine antibody on cultured A549 human lung adenocarcinoma epithelial cells infected with different influenza virus strains indicated acetylation of the viral NP. A series of biochemical analyses disclosed that the host lysine acetyltransferases GCN5 and PCAF acetylate NP
収録刊行物
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- Journal of Biological Chemistry
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Journal of Biological Chemistry 293 (19), 7126-7138, 2018-05
Elsevier BV
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キーワード
- Transcription, Genetic
- Lysine
- Viral Core Proteins
- Blotting, Western
- RNA-Binding Proteins
- Acetylation
- Epithelial Cells
- Nucleocapsid Proteins
- Virus Replication
- Microbiology
- Epigenesis, Genetic
- A549 Cells
- Influenza A virus
- Tandem Mass Spectrometry
- Humans
- RNA, Viral
- RNA Interference
- p300-CBP Transcription Factors
- Amino Acid Sequence
- Protein Processing, Post-Translational
- Chromatography, Liquid
- Histone Acetyltransferases
詳細情報 詳細情報について
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- CRID
- 1360004233987978752
-
- ISSN
- 00219258
-
- PubMed
- 29555684
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- 資料種別
- journal article
-
- データソース種別
-
- Crossref
- KAKEN
- OpenAIRE
