Development of cyanine probes with dinitrobenzene quencher for rapid fluorogenic protein labelling
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- Yuichiro Hori
- Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan
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- Shinya Hirayama
- Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan
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- Kazuya Kikuchi
- Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan
Description
<jats:p>A multicolour protein labelling technique using a protein tag and fluorogenic probes is a powerful approach for spatio-temporal analyses of proteins in living cells. Since cyanine fluorophores have attractive properties for multicolour imaging of proteins, there is a huge demand to develop fluorogenic cyanine probes for specific protein labelling in living cells. Herein, we develop fluorogenic cyanine probes for labelling a protein tag by using a dinitrobenzene fluorescence quencher. The probes enhanced fluorescence intensity upon labelling reactions and emitted orange or far-red fluorescence. Intramolecular interactions between the cyanine fluorophores and the dinitrobenzene quencher led not only to fluorescence quenching of the probes in the free state but also to promotion of labelling reactions. Furthermore, the probes successfully imaged cell-surface proteins without a washing process. These findings offer valuable information on the design of fluorogenic cyanine probes and indicate that the probes are useful as novel live-cell imaging tools.</jats:p> <jats:p>This article is part of the themed issue ‘Challenges for chemistry in molecular imaging’.</jats:p>
Journal
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- Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences
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Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences 375 (2107), 20170018-, 2017-10-16
The Royal Society