A novel environmental <scp>DNA</scp> approach to quantify the cryptic invasion of non‐native genotypes
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- Kimiko Uchii
- Faculty of Pharmacy Osaka Ohtani University 3‐11‐1 Nishikiori‐kita Tondabayashi 584‐8540 Japan
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- Hideyuki Doi
- Institute for Sustainable Sciences and Development Hiroshima University 1‐3‐1 Kagamiyama Higashi‐Hiroshima 739‐8530 Japan
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- Toshifumi Minamoto
- Graduate School of Human Development and Environment Kobe University 3‐11 Tsurukabuto Nada‐ku Kobe 657‐8501 Japan
書誌事項
- 公開日
- 2015-09-08
- 資源種別
- journal article
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1111/1755-0998.12460
- 公開者
- Wiley
この論文をさがす
説明
<jats:title>Abstract</jats:title><jats:p>The invasion of non‐native species that are closely related to native species can lead to competitive elimination of the native species and/or genomic extinction through hybridization. Such invasions often become serious before they are detected, posing unprecedented threats to biodiversity. A Japanese native strain of common carp (<jats:italic>Cyprinus carpio</jats:italic>) has become endangered owing to the invasion of non‐native strains introduced from the Eurasian continent. Here, we propose a rapid environmental <jats:styled-content style="fixed-case">DNA</jats:styled-content>‐based approach to quantitatively monitor the invasion of non‐native genotypes. Using this system, we developed a method to quantify the relative proportion of native and non‐native <jats:styled-content style="fixed-case">DNA</jats:styled-content> based on a single‐nucleotide polymorphism using cycling probe technology in real‐time <jats:styled-content style="fixed-case">PCR</jats:styled-content>. The efficiency of this method was confirmed in aquarium experiments, where the quantified proportion of native and non‐native <jats:styled-content style="fixed-case">DNA</jats:styled-content> in the water was well correlated to the biomass ratio of native and non‐native genotypes. This method provided quantitative estimates for the proportion of native and non‐native <jats:styled-content style="fixed-case">DNA</jats:styled-content> in natural rivers and reservoirs, which allowed us to estimate the degree of invasion of non‐native genotypes without catching and analysing individual fish. Our approach would dramatically facilitate the process of quantitatively monitoring the invasion of non‐native conspecifics in aquatic ecosystems, thus revealing a promising method for risk assessment and management in biodiversity conservation.</jats:p>
収録刊行物
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- Molecular Ecology Resources
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Molecular Ecology Resources 16 (2), 415-422, 2015-09-08
Wiley