<i>Lactobacillus rhamnosus</i>GG SpaC pilin subunit binds to the carbohydrate moieties of intestinal glycoconjugates
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- Keita Nishiyama
- Department of Animal Science, School of Veterinary Medicine; Kitasato University; Towada
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- Shintaro Ueno
- Department of Animal Science, School of Veterinary Medicine; Kitasato University; Towada
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- Makoto Sugiyama
- Faculty of Veterinary Medicine, School of Veterinary Medicine; Kitasato University; Towada, Aomori Japan
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- Yuji Yamamoto
- Department of Animal Science, School of Veterinary Medicine; Kitasato University; Towada
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- Takao Mukai
- Department of Animal Science, School of Veterinary Medicine; Kitasato University; Towada
書誌事項
- タイトル別名
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- SPAC BINDS TO INTESTINAL GLYCOCONJUGATES
- 公開日
- 2015-10-05
- 資源種別
- journal article
- 権利情報
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- http://doi.wiley.com/10.1002/tdm_license_1.1
- DOI
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- 10.1111/asj.12491
- 公開者
- Wiley
この論文をさがす
説明
Lactobacillus rhamnosus GG (LGG) is a well-established probiotic strain. The beneficial properties of this strain are partially dependent on its prolonged residence in the gastrointestinal tract, and are likely influenced by its adhesion to the intestinal mucosa. The pilin SpaC subunit, located within the Spa pili structure, is the most well studied LGG adhesion factor. However, the binding epitopes of SpaC remain largely unknown. The aim of this study was to evaluate the binding properties of SpaC to the carbohydrate moieties of intestinal glycoconjugates using a recombinant SpaC protein. In a competitive enzyme-linked immunosorbent assay, SpaC binding was markedly reduced by addition of purified mucin and the mucin oligosaccharide fraction. Histochemical staining revealed that the binding of SpaC was drastically reduced by periodic acid treatment. Moreover, in the surface plasmon resonance-based Biacore assay, SpaC bound strongly to the carbohydrate moieties containing β-galactoside at the non-reducing terminus of glycolipids. We here provide the first demonstration that SpaC binds to the oligosaccharide chains of mucins, and that the carbohydrate moieties containing β-galactoside at the non-reducing termini of glycoconjugates play a crucial role in this binding. Our results demonstrate the importance of carbohydrates of SpaC for mucus interactions.
収録刊行物
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- Animal Science Journal
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Animal Science Journal 87 (6), 809-815, 2015-10-05
Wiley