Activated Cdc42-Bound IQGAP1 Determines the Cellular Endocytic Site
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- Toshihide Kimura
- Department of Pharmacology, Oita University Faculty of Medicine, Hasama, Yufu, Oita, Japan
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- Mami Yamaoka
- Department of Pharmacology, Oita University Faculty of Medicine, Hasama, Yufu, Oita, Japan
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- Shigeki Taniguchi
- Department of Pharmacology, Oita University Faculty of Medicine, Hasama, Yufu, Oita, Japan
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- Mitsuhiro Okamoto
- Department of Pharmacology, Oita University Faculty of Medicine, Hasama, Yufu, Oita, Japan
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- Masahiro Takei
- Department of Pharmacology, Oita University Faculty of Medicine, Hasama, Yufu, Oita, Japan
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- Tomomi Ando
- Department of Pharmacology, Oita University Faculty of Medicine, Hasama, Yufu, Oita, Japan
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- Akihiro Iwamatsu
- Protein Research Network, Inc., Midori-ku, Yokohama, Japan
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- Takashi Watanabe
- Department of Cell Pharmacology, Graduate School of Medicine, Nagoya University, Showa, Nagoya, Aichi, Japan
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- Kozo Kaibuchi
- Department of Cell Pharmacology, Graduate School of Medicine, Nagoya University, Showa, Nagoya, Aichi, Japan
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- Toshimasa Ishizaki
- Department of Pharmacology, Oita University Faculty of Medicine, Hasama, Yufu, Oita, Japan
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- Ichiro Niki
- Department of Pharmacology, Oita University Faculty of Medicine, Hasama, Yufu, Oita, Japan
書誌事項
- 公開日
- 2013-12-01
- 資源種別
- journal article
- 権利情報
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- https://journals.asm.org/non-commercial-tdm-license
- DOI
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- 10.1128/mcb.00895-13
- 公開者
- Informa UK Limited
説明
Recruitment of specific molecules to a specific membrane site is essential for communication between specialized membranous organelles. In the present study, we identified IQGAP1 as a novel GDP-bound-Rab27a-interacting protein. We found that IQGAP1 interacts with GDP-bound Rab27a when it forms a complex with GTP-bound Cdc42. We also found that IQGAP1 regulates the endocytosis of insulin secretory membranes. Silencing of IQGAP1 inhibits both endocytosis and the glucose-induced redistribution of endocytic machinery, including Rab27a and its binding protein coronin 3. These processes can also be inhibited by disruption of the trimeric complex with dominant negative IQGAP1 and Cdc42. These results indicate that activation of Cdc42 in response to the insulin secretagogue glucose recruits endocytic machinery to IQGAP1 at the cell periphery and regulates endocytosis at this membrane site.
収録刊行物
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- Molecular and Cellular Biology
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Molecular and Cellular Biology 33 (24), 4834-4843, 2013-12-01
Informa UK Limited
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キーワード
- Male
- Guanosine Diphosphate
- rab27 GTP-Binding Proteins
- Mice
- Cell Line, Tumor
- Chlorocebus aethiops
- Insulin Secretion
- Animals
- Humans
- Insulin
- cdc42 GTP-Binding Protein
- Pancreas
- Mice, Inbred ICR
- Cell Membrane
- Microfilament Proteins
- Endocytosis
- Protein Transport
- Glucose
- rab GTP-Binding Proteins
- ras GTPase-Activating Proteins
- COS Cells
- Protein Multimerization
- Protein Binding
詳細情報 詳細情報について
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- CRID
- 1360004235993734784
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- ISSN
- 10985549
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- PubMed
- 24100016
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- 資料種別
- journal article
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- データソース種別
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- Crossref
- KAKEN
- OpenAIRE
