Regulation of Epstein-Barr Virus Life Cycle and Cell Proliferation by Histone H3K27 Methyltransferase EZH2 in Akata Cells
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- Takaya Ichikawa
- Department of Virology, Nagoya University Graduate School of Medicine, Nagoya, Japan
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- Yusuke Okuno
- Center for Advanced Medicine and Clinical Research, Nagoya University Hospital, Nagoya, Japan
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- Yoshitaka Sato
- Department of Virology, Nagoya University Graduate School of Medicine, Nagoya, Japan
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- Fumi Goshima
- Department of Virology, Nagoya University Graduate School of Medicine, Nagoya, Japan
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- Hironori Yoshiyama
- Department of Microbiology, Shimane University Faculty of Medicine, Izumo, Japan
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- Teru Kanda
- Division of Microbiology, Faculty of Medicine, Tohoku Medical and Pharmaceutical University, Sendai, Japan
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- Hiroshi Kimura
- Department of Virology, Nagoya University Graduate School of Medicine, Nagoya, Japan
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- Takayuki Murata
- Department of Virology, Nagoya University Graduate School of Medicine, Nagoya, Japan
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- Blossom Damania
- editor
Description
<jats:p>The life cycle of EBV is regulated by epigenetic modifications, such as CpG methylation and histone modifications. Here, we found that the expression of EZH2, which encodes a histone H3K27 methyltransferase, was induced by EBV infection; therefore, we generated EZH2-KO cells to investigate the role of EZH2 in EBV-infected Akata B cells. Disruption of EZH2 resulted in increased expression of EBV genes during the lytic phase and, therefore, efficient viral replication and progeny production. Our results shed light on the mechanisms underlying reactivation from an epigenetic point of view and further suggest a role for EZH2 as a form of innate immunity that restricts viral replication in infected cells.</jats:p>
Journal
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- mSphere
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mSphere 3 (6), e00478-18-, 2018-12-26
American Society for Microbiology
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Keywords
- Gene Expression Regulation, Viral
- EBV; EZH2; histone methylation
- Herpesvirus 4, Human
- Virus Replication
- Microbiology
- QR1-502
- Cell Line
- Epigenesis, Genetic
- Host-Microbe Biology
- Gene Knockout Techniques
- EBV
- Host-Pathogen Interactions
- Humans
- Enhancer of Zeste Homolog 2 Protein
- EZH2
- histone methylation
- Gene Deletion
- Cell Proliferation
- Research Article
Details 詳細情報について
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- CRID
- 1360004235997513984
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- ISSN
- 23795042
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- PubMed
- 30487153
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- Data Source
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- Crossref
- KAKEN
- OpenAIRE