Inhibition of Prolyl Hydroxylase Attenuates Fas Ligand–Induced Apoptosis and Lung Injury in Mice
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- Yusuke Nagamine
- Department of Anesthesiology and Critical Care Medicine, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan
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- Kentaro Tojo
- Department of Anesthesiology and Critical Care Medicine, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan
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- Takuya Yazawa
- Department of Diagnostic Pathology, Chiba University Graduate School of Medicine, Chiba, Chiba, Japan; and
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- Shunsuke Takaki
- Department of Anesthesiology and Critical Care Medicine, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan
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- Yasuko Baba
- Operation Department, Yokohama City University Medical Center, Yokohama, Kanagawa, Japan
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- Takahisa Goto
- Department of Anesthesiology and Critical Care Medicine, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan
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- Kiyoyasu Kurahashi
- Department of Anesthesiology and Critical Care Medicine, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan
書誌事項
- 公開日
- 2016-12-01
- 資源種別
- journal article
- 権利情報
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- https://academic.oup.com/pages/standard-publication-reuse-rights
- DOI
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- 10.1165/rcmb.2015-0266oc
- 公開者
- Oxford University Press (OUP)
この論文をさがす
説明
<jats:title>Abstract</jats:title> <jats:p>Alveolar epithelial injury and increased alveolar permeability are hallmarks of acute respiratory distress syndrome. Apoptosis of lung epithelial cells via the Fas/Fas ligand (FasL) pathway plays a critical role in alveolar epithelial injury. Activation of hypoxia-inducible factor (HIF)-1 by inhibition of prolyl hydroxylase domain proteins (PHDs) is a possible therapeutic approach to attenuate apoptosis and organ injury. Here, we investigated whether treatment with dimethyloxalylglycine (DMOG), an inhibitor of PHDs, could attenuate Fas/FasL–dependent apoptosis in lung epithelial cells and lung injury. DMOG increased HIF-1α protein expression in vitro in MLE-12 cells, a murine alveolar epithelial cell line. Treatment of MLE-12 cells with DMOG significantly suppressed cell surface expression of Fas and attenuated FasL-induced caspase-3 activation and apoptotic cell death. Inhibition of the HIF-1 pathway by echinomycin or small interfering RNA transfection abolished these antiapoptotic effects of DMOG. Moreover, intraperitoneal injection of DMOG in mice increased HIF-1α expression and decreased Fas expression in lung tissues. DMOG treatment significantly attenuated caspase-3 activation, apoptotic cell death in lung tissue, and the increase in alveolar permeability in mice instilled intratracheally with FasL. In addition, inflammatory responses and histopathological changes were also significantly attenuated by DMOG treatment. In conclusion, inhibition of PHDs protects lung epithelial cells from Fas/FasL–dependent apoptosis through HIF-1 activation and attenuates lung injury in mice.</jats:p>
収録刊行物
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- American Journal of Respiratory Cell and Molecular Biology
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American Journal of Respiratory Cell and Molecular Biology 55 (6), 878-888, 2016-12-01
Oxford University Press (OUP)
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キーワード
- Male
- Cell Membrane Permeability
- Fas Ligand Protein
- Caspase 3
- Protein Stability
- Fas-Associated Death Domain Protein
- Procollagen-Proline Dioxygenase
- Apoptosis
- Lung Injury
- Hypoxia-Inducible Factor 1, alpha Subunit
- Amino Acids, Dicarboxylic
- Cell Line
- Mice, Inbred C57BL
- Animals
- Humans
- Lung
- Signal Transduction
詳細情報 詳細情報について
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- CRID
- 1360004236537670656
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- ISSN
- 15354989
- 10441549
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- PubMed
- 27494234
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- 資料種別
- journal article
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- データソース種別
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- Crossref
- KAKEN
- OpenAIRE
