Enhanced Beetle Luciferase for High-Resolution Bioluminescence Imaging
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- Immo A. Hansen
- editor
書誌事項
- 公開日
- 2010-04-02
- 資源種別
- journal article
- 権利情報
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- http://creativecommons.org/licenses/by/4.0/
- DOI
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- 10.1371/journal.pone.0010011
- 公開者
- Public Library of Science (PLoS)
説明
We developed an enhanced green-emitting luciferase (ELuc) to be used as a bioluminescence imaging (BLI) probe. ELuc exhibits a light signal in mammalian cells that is over 10-fold stronger than that of the firefly luciferase (FLuc), which is the most widely used luciferase reporter gene. We showed that ELuc produces a strong light signal in primary cells and tissues and that it enables the visualization of gene expression with high temporal resolution at the single-cell level. Moreover, we successfully imaged the nucleocytoplasmic shuttling of importin alpha by fusing ELuc at the intracellular level. These results demonstrate that the use of ELuc allows a BLI spatiotemporal resolution far greater than that provided by FLuc.
収録刊行物
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- PLoS ONE
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PLoS ONE 5 (4), e10011-, 2010-04-02
Public Library of Science (PLoS)
