Deep sequencing-based microRNA expression signatures in head and neck squamous cell carcinoma: dual strands of pre-<i>miR</i>-150 as antitumor miRNAs
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- Keiichi Koshizuka
- Department of Functional Genomics, Chiba University Graduate School of Medicine, Chuo-Ku, Chiba, Japan
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- Nijiro Nohata
- Moores Cancer Center, University of California, San Diego, La Jolla, CA, USA
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- Toyoyuki Hanazawa
- Department of Otorhinolaryngology/Head and Neck Surgery, Chiba University Graduate School of Medicine, Chiba, Japan
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- Naoko Kikkawa
- Department of Otorhinolaryngology/Head and Neck Surgery, Chiba University Graduate School of Medicine, Chiba, Japan
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- Takayuki Arai
- Department of Functional Genomics, Chiba University Graduate School of Medicine, Chuo-Ku, Chiba, Japan
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- Atsushi Okato
- Department of Functional Genomics, Chiba University Graduate School of Medicine, Chuo-Ku, Chiba, Japan
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- Ichiro Fukumoto
- Department of Functional Genomics, Chiba University Graduate School of Medicine, Chuo-Ku, Chiba, Japan
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- Koji Katada
- Department of Otorhinolaryngology/Head and Neck Surgery, Chiba University Graduate School of Medicine, Chiba, Japan
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- Yoshitaka Okamoto
- Department of Otorhinolaryngology/Head and Neck Surgery, Chiba University Graduate School of Medicine, Chiba, Japan
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- Naohiko Seki
- Department of Functional Genomics, Chiba University Graduate School of Medicine, Chuo-Ku, Chiba, Japan
書誌事項
- 公開日
- 2017-03-17
- 資源種別
- journal article
- DOI
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- 10.18632/oncotarget.16327
- 公開者
- Impact Journals, LLC
説明
We adopted into RNA-sequencing technologies to construct the microRNA (miRNA) expression signature of head and neck squamous cell carcinoma (HNSCC). Our signature revealed that a total of 160 miRNAs (44 upregulated and 116 downregulated) were aberrantly expressed in cancer tissues. Expression of miR-150-5p (guide strand miRNA) and miR-150-3p (passenger strand miRNA) were significantly silenced in cancer tissues, suggesting both miRNAs act as antitumor miRNAs in HNSCC cells. Ectopic expression of mature miRNAs, miR-150-5p and miR-150-3p inhibited cancer cell aggressiveness. Low expression of miR-150-5p and miR-150-3p predicted significantly shorter overall survival in patients with HNSCC (P = 0.0091 and P = 0.0386) by Kaplan-Meier survival curves analyses. We identified that integrin α3 (ITGA3), integrin α6 (ITGA6), and tenascin C (TNC) were coordinately regulated by these miRNAs in HNSCC cells. Knockdown assays using siRNAs showed that ITGA3, ITGA6 and TNC acted as cancer promoting genes in HNSCC cells. Moreover, ITGA3, ITGA6, and TNC alterations were associated with significantly poorer overall survival (P = 0.0177, P = 0.0237, and P = 0.026, respectively). Dual strands of pre-150 (miR-150-5p and miR-150-3p) functioned as antitumor miRNAs based on the miRNA expression signature of HNSCC. Identification of antitumor miR-150-mediated RNA networks may provide novel insights into pathogenesis of HNSCC.
収録刊行物
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- Oncotarget
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Oncotarget 8 (18), 30288-30304, 2017-03-17
Impact Journals, LLC
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キーワード
- Aged, 80 and over
- Male
- Gene Expression Profiling
- High-Throughput Nucleotide Sequencing
- Middle Aged
- Ectopic Gene Expression
- Gene Expression Regulation, Neoplastic
- MicroRNAs
- Cell Movement
- Head and Neck Neoplasms
- Cell Line, Tumor
- Gene Knockdown Techniques
- Biomarkers, Tumor
- Carcinoma, Squamous Cell
- Humans
- Female
- Neoplasm Grading
- Research Paper
- Aged
- Cell Proliferation
- Neoplasm Staging
詳細情報 詳細情報について
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- CRID
- 1360004238271636736
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- ISSN
- 19492553
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- PubMed
- 28415821
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- 資料種別
- journal article
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- データソース種別
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- Crossref
- KAKEN
- OpenAIRE