Boosting Sensitivity in Liquid Chromatography–Fourier Transform Ion Cyclotron Resonance–Tandem Mass Spectrometry for Product Ion Analysis of Monoterpene Indole Alkaloids
書誌事項
- 公開日
- 2015-12-17
- 資源種別
- journal article
- DOI
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- 10.3389/fpls.2015.01127
- 公開者
- Frontiers Media SA
説明
In metabolomics, the analysis of product ions in tandem mass spectrometry (MS/MS) is noteworthy to chemically assign structural information. However, the development of relevant analytical methods are less advanced. Here, we developed a method to boost sensitivity in liquid chromatography-Fourier transform ion cyclotron resonance-tandem mass spectrometry analysis (MS/MS boost analysis). To verify the MS/MS boost analysis, both quercetin and uniformly labeled (13)C quercetin were analyzed, revealing that the origin of the product ions is not the instrument, but the analyzed compounds resulting in sensitive product ions. Next, we applied this method to the analysis of monoterpene indole alkaloids (MIAs). The comparative analyses of MIAs having indole basic skeleton (ajmalicine, catharanthine, hirsuteine, and hirsutine) and oxindole skeleton (formosanine, isoformosanine, pteropodine, isopteropodine, rhynchophylline, isorhynchophylline, and mitraphylline) identified 86 and 73 common monoisotopic ions, respectively. The comparative analyses of the three pairs of stereoisomers showed more than 170 common monoisotopic ions in each pair. This method was also applied to the targeted analysis of MIAs in Catharanthus roseus and Uncaria rhynchophylla to profile indole and oxindole compounds using the product ions. This analysis is suitable for chemically assigning features of the metabolite groups, which contributes to targeted metabolome analysis.
収録刊行物
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- Frontiers in Plant Science
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Frontiers in Plant Science 6 2015-12-17
Frontiers Media SA
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キーワード
- MIAtargeted analysis
- MIA-targeted analysis
- Plant culture
- monoterpene indole alkaloid (MIA)
- Plant Science
- metabolomics
- SB1-1110
- Tandem Mass Spectrometry
- tandem mass spectrometry
- Metabolomics
- liquid chromatography–Fourier transform ion cyclotron resonance–tandem mass spectrometry
- specialized metabolite
詳細情報 詳細情報について
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- CRID
- 1360004239462065280
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- ISSN
- 1664462X
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- PubMed
- 26734034
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- 資料種別
- journal article
-
- データソース種別
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- Crossref
- KAKEN
- OpenAIRE
