Protein Expression Analyses at the Single Cell Level

DOI Web Site 参考文献64件 オープンアクセス
  • Masae Ohno
    Laboratory for Single Cell Gene Dynamics, Quantitative Biology Center, RIKEN Address, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan
  • Peter Karagiannis
    Laboratory for Single Cell Gene Dynamics, Quantitative Biology Center, RIKEN Address, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan
  • Yuichi Taniguchi
    Laboratory for Single Cell Gene Dynamics, Quantitative Biology Center, RIKEN Address, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan

書誌事項

公開日
2014-09-05
資源種別
journal article
権利情報
  • https://creativecommons.org/licenses/by/3.0/
DOI
  • 10.3390/molecules190913932
公開者
MDPI AG

説明

<jats:p>The central dogma of molecular biology explains how genetic information is converted into its end product, proteins, which are responsible for the phenotypic state of the cell. Along with the protein type, the phenotypic state depends on the protein copy number. Therefore, quantification of the protein expression in a single cell is critical for quantitative characterization of the phenotypic states. Protein expression is typically a dynamic and stochastic phenomenon that cannot be well described by standard experimental methods. As an alternative, fluorescence imaging is being explored for the study of protein expression, because of its high sensitivity and high throughput. Here we review key recent progresses in fluorescence imaging-based methods and discuss their application to proteome analysis at the single cell level.</jats:p>

収録刊行物

  • Molecules

    Molecules 19 (9), 13932-13947, 2014-09-05

    MDPI AG

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