Uptake of osteoblast-derived extracellular vesicles promotes the differentiation of osteoclasts in the zebrafish scale
書誌事項
- 公開日
- 2020-04-23
- 資源種別
- journal article
- 権利情報
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- https://creativecommons.org/licenses/by/4.0
- https://creativecommons.org/licenses/by/4.0
- DOI
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- 10.1038/s42003-020-0925-1
- 公開者
- Springer Science and Business Media LLC
説明
<jats:title>Abstract</jats:title><jats:p>Differentiation of osteoclasts (OCs) from hematopoietic cells requires cellular interaction with osteoblasts (OBs). Due to the difficulty of live-imaging in the bone, however, the cellular and molecular mechanisms underlying intercellular communication involved in OC differentiation are still elusive. Here, we develop a fracture healing model using the scale of <jats:italic>trap:GFP</jats:italic>; <jats:italic>osterix:mCherry</jats:italic> transgenic zebrafish to visualize the interaction between OCs and OBs. Transplantation assays followed by flow cytometric analysis reveal that most <jats:italic>trap:GFP</jats:italic><jats:sup>high</jats:sup> OCs in the fractured scale are detected in the <jats:italic>osterix:mCherry</jats:italic><jats:sup>+</jats:sup> fraction because of uptake of OB-derived extracellular vesicles (EVs). In vivo live-imaging shows that immature OCs actively interact with <jats:italic>osterix:mCherry</jats:italic><jats:sup>+</jats:sup> OBs and engulf EVs prior to convergence at the fracture site. In vitro cell culture assays show that OB-derived EVs promote OC differentiation via Rankl signaling. Collectively, these data suggest that EV-mediated intercellular communication with OBs plays an important role in the differentiation of OCs in bone tissue.</jats:p>
収録刊行物
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- Communications Biology
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Communications Biology 3 (1), 190-, 2020-04-23
Springer Science and Business Media LLC