Cutting the ovarian surface improves the responsiveness to exogenous hormonal treatment in aged mice
-
- Takashi Umehara
- Graduate School of Integrated Sciences for Life Hiroshima University Higashi‐Hiroshima Japan
-
- Nao Urabe
- Saint Mother Obstetrics and Gynecology Clinic Institute for ART Fukuoka Japan
-
- Toshiki Obata
- Saint Mother Obstetrics and Gynecology Clinic Institute for ART Fukuoka Japan
-
- Takashi Yamaguchi
- Saint Mother Obstetrics and Gynecology Clinic Institute for ART Fukuoka Japan
-
- Atsushi Tanaka
- Saint Mother Obstetrics and Gynecology Clinic Institute for ART Fukuoka Japan
-
- Masayuki Shimada
- Graduate School of Integrated Sciences for Life Hiroshima University Higashi‐Hiroshima Japan
この論文をさがす
説明
<jats:title>Abstract</jats:title><jats:sec><jats:title>Purpose</jats:title><jats:p>Ovarian vascular abnormality and ovarian fibrosis are observed in the low responder patients and aging mice. Vascularization and fibrosis are regulated by injury‐repair system, such as wound. Thus, in this study, the authors tried to investigate the effect of the surgical treatment to ovarian surface with cutting on the functions of ovary in aging mouse model, gc<jats:italic>Nrg1</jats:italic>KO.</jats:p></jats:sec><jats:sec><jats:title>Method</jats:title><jats:p>The ovarian surface of gc<jats:italic>Nrg1</jats:italic>KO was surgically cut, and then the ovary was returned inside of bursa ovarica. To assess the effect of cutting on fertility, mating test, smear analysis, and exogenous hormonal treatment were done. Additionally, the histological analysis was used for observing the remodeling of ovarian stroma after the surgical approach.</jats:p></jats:sec><jats:sec><jats:title>Result</jats:title><jats:p>Ovarian fibrosis disappeared at 7 days after surgery. With the abrogation of fibrosis, the blood vessels were fluently observed around the follicles, and the follicular development was re‐started. The responses against exogenous hormone were recovered at 21 days after the surgery, and estrous cycle and delivery were also recovered by the surgery and the fertility was maintained for 3 months.</jats:p></jats:sec><jats:sec><jats:title>Conclusion</jats:title><jats:p>This cutting method of ovarian surface becomes a good option against low responder patients.</jats:p></jats:sec>
収録刊行物
-
- Reproductive Medicine and Biology
-
Reproductive Medicine and Biology 19 (4), 415-424, 2020-08-26
Wiley