Characterization of mammalian orthoreoviruses isolated from faeces of pigs in Zambia

DOI Web Site 被引用文献5件 参考文献50件 オープンアクセス
  • Hayato Harima
    Hokudai Center for Zoonosis Control in Zambia, School of Veterinary Medicine, the University of Zambia, PO Box 32379, Lusaka, 10101, Zambia
  • Michihito Sasaki
    Division of Molecular Pathobiology, Hokkaido University, Research Center for Zoonosis Control, N20 W10, Kita-ku, Sapporo, 001-0020, Japan
  • Masahiro Kajihara
    Division of Global Epidemiology, Hokkaido University, Research Center for Zoonosis Control, N20 W10, Kita-ku, Sapporo, 001-0020, Japan
  • Gabriel Gonzalez
    Division of Bioinformatics, Hokkaido University, Research Center for Zoonosis Control, N20 W10, Kita-ku, Sapporo, 001-0020, Japan
  • Edgar Simulundu
    Department of Disease Control, School of Veterinary Medicine, the University of Zambia, PO Box 32379, Lusaka, 10101, Zambia
  • Eugene C. Bwalya
    Department of Clinical Studies, School of Veterinary Medicine, the University of Zambia, PO Box 32379, Lusaka, 10101, Zambia
  • Yongjin Qiu
    Hokudai Center for Zoonosis Control in Zambia, School of Veterinary Medicine, the University of Zambia, PO Box 32379, Lusaka, 10101, Zambia
  • Kosuke Okuya
    Division of Global Epidemiology, Hokkaido University, Research Center for Zoonosis Control, N20 W10, Kita-ku, Sapporo, 001-0020, Japan
  • Mao Isono
    Division of Global Epidemiology, Hokkaido University, Research Center for Zoonosis Control, N20 W10, Kita-ku, Sapporo, 001-0020, Japan
  • Yasuko Orba
    Division of Molecular Pathobiology, Hokkaido University, Research Center for Zoonosis Control, N20 W10, Kita-ku, Sapporo, 001-0020, Japan
  • Ayato Takada
    Division of Global Epidemiology, Hokkaido University, Research Center for Zoonosis Control, N20 W10, Kita-ku, Sapporo, 001-0020, Japan
  • Bernard M. Hang’ombe
    Department of Para-clinical Studies, School of Veterinary Medicine, the University of Zambia, PO Box 32379, Lusaka, 10101, Zambia
  • Aaron S. Mweene
    Africa Center of Excellence for Infectious Diseases of Humans and Animals, the University of Zambia, PO Box 32379, Lusaka, 10101, Zambia
  • Hirofumi Sawa
    Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University Kita-ku, Sapporo, 001-0020, Japan

説明

<jats:p>Mammalian orthoreovirus (MRV) has been identified in humans, livestock and wild animals; this wide host range allows individual MRV to transmit into multiple species. Although several interspecies transmission and genetic reassortment events of MRVs among humans, livestock and wildlife have been reported, the genetic diversity and geographic distribution of MRVs in Africa are poorly understood. In this study, we report the first isolation and characterization of MRVs circulating in a pig population in Zambia. In our screening, MRV genomes were detected in 19.7 % (29/147) of faecal samples collected from pigs by reverse transcription PCR. Three infectious MRV strains (MRV-85, MRV-96 and MRV-117) were successfully isolated, and their complete genomes were sequenced. Recombination analyses based on the complete genome sequences of the isolated MRVs demonstrated that MRV-96 shared the S3 segment with a different MRV isolated from bats, and that the L1 and M3 segments of MRV-117 originated from bat and human MRVs, respectively. Our results suggest that the isolated MRVs emerged through genetic reassortment events with interspecies transmission. Given the lack of information regarding MRVs in Africa, further surveillance of MRVs circulating among humans, domestic animals and wildlife is required to assess potential risk for humans and animals.</jats:p>

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