A distinct “side population” of cells with high drug efflux capacity in human tumor cells

DOI Web Site 被引用文献47件
  • C. Hirschmann-Jax
    Center for Cell and Gene Therapy, Baylor College of Medicine, Methodist Hospital and Texas Children's Hospital, and DeBakey Department of Surgery, One Baylor Plaza, Houston, TX 77030; Department of Pediatric Hematology and Oncology, Heinrich-Heine-University Medical Center, D-40225 Dusseldorf, Germany; and Department of Internal Medicine, Heinrich-Heine-University Medical Center, D-40225 Dusseldorf, Germany
  • A. E. Foster
    Center for Cell and Gene Therapy, Baylor College of Medicine, Methodist Hospital and Texas Children's Hospital, and DeBakey Department of Surgery, One Baylor Plaza, Houston, TX 77030; Department of Pediatric Hematology and Oncology, Heinrich-Heine-University Medical Center, D-40225 Dusseldorf, Germany; and Department of Internal Medicine, Heinrich-Heine-University Medical Center, D-40225 Dusseldorf, Germany
  • G. G. Wulf
    Center for Cell and Gene Therapy, Baylor College of Medicine, Methodist Hospital and Texas Children's Hospital, and DeBakey Department of Surgery, One Baylor Plaza, Houston, TX 77030; Department of Pediatric Hematology and Oncology, Heinrich-Heine-University Medical Center, D-40225 Dusseldorf, Germany; and Department of Internal Medicine, Heinrich-Heine-University Medical Center, D-40225 Dusseldorf, Germany
  • J. G. Nuchtern
    Center for Cell and Gene Therapy, Baylor College of Medicine, Methodist Hospital and Texas Children's Hospital, and DeBakey Department of Surgery, One Baylor Plaza, Houston, TX 77030; Department of Pediatric Hematology and Oncology, Heinrich-Heine-University Medical Center, D-40225 Dusseldorf, Germany; and Department of Internal Medicine, Heinrich-Heine-University Medical Center, D-40225 Dusseldorf, Germany
  • T. W. Jax
    Center for Cell and Gene Therapy, Baylor College of Medicine, Methodist Hospital and Texas Children's Hospital, and DeBakey Department of Surgery, One Baylor Plaza, Houston, TX 77030; Department of Pediatric Hematology and Oncology, Heinrich-Heine-University Medical Center, D-40225 Dusseldorf, Germany; and Department of Internal Medicine, Heinrich-Heine-University Medical Center, D-40225 Dusseldorf, Germany
  • U. Gobel
    Center for Cell and Gene Therapy, Baylor College of Medicine, Methodist Hospital and Texas Children's Hospital, and DeBakey Department of Surgery, One Baylor Plaza, Houston, TX 77030; Department of Pediatric Hematology and Oncology, Heinrich-Heine-University Medical Center, D-40225 Dusseldorf, Germany; and Department of Internal Medicine, Heinrich-Heine-University Medical Center, D-40225 Dusseldorf, Germany
  • M. A. Goodell
    Center for Cell and Gene Therapy, Baylor College of Medicine, Methodist Hospital and Texas Children's Hospital, and DeBakey Department of Surgery, One Baylor Plaza, Houston, TX 77030; Department of Pediatric Hematology and Oncology, Heinrich-Heine-University Medical Center, D-40225 Dusseldorf, Germany; and Department of Internal Medicine, Heinrich-Heine-University Medical Center, D-40225 Dusseldorf, Germany
  • M. K. Brenner
    Center for Cell and Gene Therapy, Baylor College of Medicine, Methodist Hospital and Texas Children's Hospital, and DeBakey Department of Surgery, One Baylor Plaza, Houston, TX 77030; Department of Pediatric Hematology and Oncology, Heinrich-Heine-University Medical Center, D-40225 Dusseldorf, Germany; and Department of Internal Medicine, Heinrich-Heine-University Medical Center, D-40225 Dusseldorf, Germany

説明

<jats:p> A subset of stem cells, termed the “side population” (SP), has been identified in several tissues in mammalian species. These cells maintain a high efflux capability for antimitotic drugs. We have investigated whether functionally equivalent stem cells also may be detected in human cancers. We initially examined primary tumor cells from 23 patients with neuroblastoma and cell lines derived from a range of other tumors. A distinct SP was found in neuroblastoma cells from 15 of 23 patients (65%). The SP was capable of sustained expansion <jats:italic>ex vivo</jats:italic> and showed evidence for asymmetric division, generating both SP and non-SP progeny. These cells also expressed high levels of <jats:italic>ABCG2</jats:italic> and <jats:italic>ABCA3</jats:italic> transporter genes and had a greater capacity to expel cytotoxic drugs, such as mitoxantrone, resulting in better survival. A SP also was detected in breast cancer, lung cancer, and glioblastoma cell lines, suggesting that this phenotype defines a class of cancer stem cells with inherently high resistance to chemotherapeutic agents that should be targeted during the treatment of malignant disease. </jats:p>

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