Standardized Method for In Vitro Antifungal Susceptibility Testing of
            <i>Candida albicans</i>
            Biofilms

Gordon Ramage, Kacy Vande Walle, Brian L. Wickes, José L. López-Ribot

doi:10.1128/aac.45.9.2475-2479.2001

<jats:title>ABSTRACT</jats:title> <jats:p> <jats:italic>Candida albicans</jats:italic> is implicated in many biomaterial-related infections. Typically, these infections are associated with biofilm formation. Cells in biofilms display phenotypic traits that are dramatically different from those of their free-floating planktonic counterparts and are notoriously resistant to antimicrobial agents. Consequently, biofilm-related infections are inherently difficult to treat and to fully eradicate with normal treatment regimens. Here, we report a rapid and highly reproducible microtiter-based colorimetric assay for the susceptibility testing of fungal biofilms, based on the measurement of metabolic activities of the sessile cells by using a formazan salt reduction assay. The assay was used for in vitro antifungal susceptibility testing of several <jats:italic>C. albicans</jats:italic> strains grown as biofilms against amphotericin B and fluconazole and the increased resistance of <jats:italic>C. albicans</jats:italic> biofilms against these antifungal agents was demonstrated. Because of its simplicity, compatibility with a widely available 96-well microplate platform, high throughput, and automation potential, we believe this assay represents a promising tool for the standardization of in vitro antifungal susceptibility testing of fungal biofilms. </jats:p>

Standardized Method for In Vitro Antifungal Susceptibility Testing of <i>Candida albicans</i> Biofilms

書誌事項

この論文をさがす

説明

収録刊行物

被引用文献 (8)*注記

詳細情報詳細情報について

書き出し

問題の指摘