Hematopoietic origin of glomerular mesangial cells

DOI PDF 被引用文献8件
  • Masahiro Masuya
    From the Department of Veterans Affairs Medical Center, Department of Medicine and Department of Cell Biology and Anatomy, Medical University of South Carolina, Charleston, SC; and the Departments of Neurology and Anatomy and Cell Biology, Medical College of Georgia and the Department of Veterans Affairs Medical Center, Augusta, GA.
  • Christopher J. Drake
    From the Department of Veterans Affairs Medical Center, Department of Medicine and Department of Cell Biology and Anatomy, Medical University of South Carolina, Charleston, SC; and the Departments of Neurology and Anatomy and Cell Biology, Medical College of Georgia and the Department of Veterans Affairs Medical Center, Augusta, GA.
  • Paul A. Fleming
    From the Department of Veterans Affairs Medical Center, Department of Medicine and Department of Cell Biology and Anatomy, Medical University of South Carolina, Charleston, SC; and the Departments of Neurology and Anatomy and Cell Biology, Medical College of Georgia and the Department of Veterans Affairs Medical Center, Augusta, GA.
  • Christopher M. Reilly
    From the Department of Veterans Affairs Medical Center, Department of Medicine and Department of Cell Biology and Anatomy, Medical University of South Carolina, Charleston, SC; and the Departments of Neurology and Anatomy and Cell Biology, Medical College of Georgia and the Department of Veterans Affairs Medical Center, Augusta, GA.
  • Haiqun Zeng
    From the Department of Veterans Affairs Medical Center, Department of Medicine and Department of Cell Biology and Anatomy, Medical University of South Carolina, Charleston, SC; and the Departments of Neurology and Anatomy and Cell Biology, Medical College of Georgia and the Department of Veterans Affairs Medical Center, Augusta, GA.
  • William D. Hill
    From the Department of Veterans Affairs Medical Center, Department of Medicine and Department of Cell Biology and Anatomy, Medical University of South Carolina, Charleston, SC; and the Departments of Neurology and Anatomy and Cell Biology, Medical College of Georgia and the Department of Veterans Affairs Medical Center, Augusta, GA.
  • Angeline Martin-Studdard
    From the Department of Veterans Affairs Medical Center, Department of Medicine and Department of Cell Biology and Anatomy, Medical University of South Carolina, Charleston, SC; and the Departments of Neurology and Anatomy and Cell Biology, Medical College of Georgia and the Department of Veterans Affairs Medical Center, Augusta, GA.
  • David C. Hess
    From the Department of Veterans Affairs Medical Center, Department of Medicine and Department of Cell Biology and Anatomy, Medical University of South Carolina, Charleston, SC; and the Departments of Neurology and Anatomy and Cell Biology, Medical College of Georgia and the Department of Veterans Affairs Medical Center, Augusta, GA.
  • Makio Ogawa
    From the Department of Veterans Affairs Medical Center, Department of Medicine and Department of Cell Biology and Anatomy, Medical University of South Carolina, Charleston, SC; and the Departments of Neurology and Anatomy and Cell Biology, Medical College of Georgia and the Department of Veterans Affairs Medical Center, Augusta, GA.

抄録

<jats:p>It was recently reported that crude bone marrow cells have the ability to differentiate into glomerular mesangial cells. However, the exact nature of the engrafting cells in the bone marrow was not known. We tested the hypothesis that hematopoietic stem cells are capable of reconstituting the mesangial cells by transplanting a clonal population of cells derived from a single stem cell. We cultured Lin−, Sca-1+, c-kit+, CD34− bone marrow cells from transgenic enhanced green fluorescent protein (EGFP) mice (C57BL/6-Ly-5.2 background) individually for 1 week in the presence of interleukin-11 and steel factor. We then transplanted viable clones individually into lethally irradiated C57BL/6-Ly-5.1 mice. Kidneys from 5 recipient mice showing high levels (60%-90%) of multilineage hematopoietic reconstitution were examined 2 to 6 months later, using differential interference contrast and epifluorescence microscopy. EGFP+ cells with a morphology characteristic of mesangial cells were evident within the glomeruli. Transplantation of 100 noncultured Lin−, Sca-1+, c-kit+, CD34− bone marrow cells also generated mesangial cells. Cultured EGFP+ glomerular cells from recipient mice contracted in response to angiotensin II. EGFP+ mesangial cells seen in male-to-male transplants revealed only one Y-chromosome. These data demonstrate that a single hematopoietic stem cell is capable of differentiating into glomerular mesangial cells and that the process does not involve cell fusion.</jats:p>

収録刊行物

  • Blood

    Blood 101 (6), 2215-2218, 2003-03-15

    American Society of Hematology

被引用文献 (8)*注記

もっと見る

詳細情報 詳細情報について

問題の指摘

ページトップへ