Bacterial Genome Editing with CRISPR-Cas9: Deletion, Integration, Single Nucleotide Modification, and Desirable “Clean” Mutant Selection in <i>Clostridium beijerinckii</i> as an Example

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  • Yi Wang
    Department of Food Science and Human Nutrition, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States
  • Zhong-Tian Zhang
    Department of Chemistry, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States
  • Seung-Oh Seo
    Department of Food Science and Human Nutrition, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States
  • Patrick Lynn
    Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States
  • Ting Lu
    Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States
  • Yong-Su Jin
    Department of Food Science and Human Nutrition, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States
  • Hans P. Blaschek
    Department of Food Science and Human Nutrition, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States

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