Enhanced cellulase production by <i>Trichoderma harzianum</i> by cultivation on glycerol followed by induction on cellulosic substrates

  • Priscila da Silva Delabona
    grid.452574.5 0000 0004 1797 1452 Brazilian Bioethanol Science and Technology Laboratory, CTBE Pólo II de Alta Tecnologia Caixa Postal 6192 Rua Giuseppe Maximo Scolfaro 10000 CEP 13083-970 Campinas SP Brazil
  • Deise Juliana Lima
    grid.452574.5 0000 0004 1797 1452 Brazilian Bioethanol Science and Technology Laboratory, CTBE Pólo II de Alta Tecnologia Caixa Postal 6192 Rua Giuseppe Maximo Scolfaro 10000 CEP 13083-970 Campinas SP Brazil
  • Diogo Robl
    grid.452574.5 0000 0004 1797 1452 Brazilian Bioethanol Science and Technology Laboratory, CTBE Pólo II de Alta Tecnologia Caixa Postal 6192 Rua Giuseppe Maximo Scolfaro 10000 CEP 13083-970 Campinas SP Brazil
  • Sarita Cândida Rabelo
    grid.452574.5 0000 0004 1797 1452 Brazilian Bioethanol Science and Technology Laboratory, CTBE Pólo II de Alta Tecnologia Caixa Postal 6192 Rua Giuseppe Maximo Scolfaro 10000 CEP 13083-970 Campinas SP Brazil
  • Cristiane Sanchez Farinas
    grid.411247.5 000000012163588X Graduate Program of Biotechnology Federal University of São Carlos 13565-905 São Carlos SP Brazil
  • José Geraldo da Cruz Pradella
    grid.452574.5 0000 0004 1797 1452 Brazilian Bioethanol Science and Technology Laboratory, CTBE Pólo II de Alta Tecnologia Caixa Postal 6192 Rua Giuseppe Maximo Scolfaro 10000 CEP 13083-970 Campinas SP Brazil

抄録

<jats:title>Abstract</jats:title> <jats:p>The use of glycerol obtained as an intermediate of the biodiesel manufacturing process as carbon source for microbial growth is a potential alternative strategy for the production of enzymes and other high-value bioproducts. This work evaluates the production of cellulase enzymes using glycerol for high cell density growth of Trichoderma harzianum followed by induction with a cellulosic material. Firstly, the influence of the carbon source used in the pre-culture step was investigated in terms of total protein secretion and fungal morphology. Enzymatic productivity was then determined for cultivation strategies using different types and concentrations of carbon source, as well as different feeding procedures (batch and fed-batch). The best strategy for cellulase production was then further studied on a larger scale using a stirred tank bioreactor. The proposed strategy for cellulase production, using glycerol to achieve high cell density growth followed by induction with pretreated sugarcane bagasse, achieved enzymatic activities up to 2.27 ± 0.37 FPU/mL, 106.40 ± 8.87 IU/mL, and 9.04 ± 0.39 IU/mL of cellulase, xylanase, and β-glucosidase, respectively. These values were 2 times higher when compared to the control experiments using glucose instead of glycerol. This novel strategy proved to be a promising approach for improving cellulolytic enzymes production, and could potentially contribute to adding value to biomass within the biofuels sector.</jats:p>

収録刊行物

被引用文献 (1)*注記

もっと見る

問題の指摘

ページトップへ