A genome-scale analysis for identification of genes required for growth or survival of <i>Haemophilus</i> <i>influenzae</i>

DOI Web Site 被引用文献8件
  • Brian J. Akerley
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109; Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115; and Department of Microbiology Molecular Genetics, Harvard Medical School, Boston, MA 02115
  • Eric J. Rubin
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109; Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115; and Department of Microbiology Molecular Genetics, Harvard Medical School, Boston, MA 02115
  • Veronica L. Novick
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109; Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115; and Department of Microbiology Molecular Genetics, Harvard Medical School, Boston, MA 02115
  • Kensey Amaya
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109; Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115; and Department of Microbiology Molecular Genetics, Harvard Medical School, Boston, MA 02115
  • Nicholas Judson
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109; Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115; and Department of Microbiology Molecular Genetics, Harvard Medical School, Boston, MA 02115
  • John J. Mekalanos
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109; Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115; and Department of Microbiology Molecular Genetics, Harvard Medical School, Boston, MA 02115

書誌事項

公開日
2002-01-22
DOI
  • 10.1073/pnas.012602299
公開者
Proceedings of the National Academy of Sciences

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説明

<jats:p> A high-density transposon mutagenesis strategy was applied to the <jats:italic>Haemophilus influenzae</jats:italic> genome to identify genes required for growth or viability. This analysis detected putative essential roles for the products of 259 ORFs of unknown function. Comparisons between complete genomes defined a subset of these proteins in <jats:italic>H. influenzae</jats:italic> having homologs in <jats:italic>Mycobacterium tuberculosis</jats:italic> that are absent in <jats:italic>Saccharomyces cerevisiae</jats:italic> , a distribution pattern that favors their use in development of antimicrobial therapeutics. Three genes within this set are essential for viability in other bacteria. Interfacing the set of essential gene products in <jats:italic>H. influenzae</jats:italic> with the distribution of homologs in other microorganisms can detect components of unrecognized cellular pathways essential in diverse bacteria. This genome-scale phenotypic analysis identifies potential roles for a large set of genes of unknown function. </jats:p>

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