Mitochondrial genome sequencing helps show the evolutionary mechanism of mitochondrial genome formation in Brassica

<jats:title>Abstract</jats:title> <jats:sec> <jats:title>Background</jats:title> <jats:p>Angiosperm mitochondrial genomes are more complex than those of other organisms. Analyses of the mitochondrial genome sequences of at least 11 angiosperm species have showed several common properties; these cannot easily explain, however, how the diverse mitotypes evolved within each genus or species. We analyzed the evolutionary relationships of <jats:italic>Brassica</jats:italic> mitotypes by sequencing.</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>We sequenced the mitotypes of <jats:italic>cam</jats:italic> (<jats:italic>Brassica rapa</jats:italic>), <jats:italic>ole</jats:italic> (<jats:italic>B. oleracea</jats:italic>), <jats:italic>jun</jats:italic> (<jats:italic>B. juncea</jats:italic>), and <jats:italic>car</jats:italic> (<jats:italic>B. carinata</jats:italic>) and analyzed them together with two previously sequenced mitotypes of <jats:italic>B</jats:italic>. <jats:italic>napus</jats:italic> (<jats:italic>pol</jats:italic> and <jats:italic>nap</jats:italic>). The sizes of whole single circular genomes of <jats:italic>cam</jats:italic>, <jats:italic>jun</jats:italic>, <jats:italic>ole</jats:italic>, and <jats:italic>car</jats:italic> are 219,747 bp, 219,766 bp, 360,271 bp, and 232,241 bp, respectively. The mitochondrial genome of <jats:italic>ole</jats:italic> is largest as a resulting of the duplication of a 141.8 kb segment. The <jats:italic>jun</jats:italic> mitotype is the result of an inherited <jats:italic>cam</jats:italic> mitotype, and <jats:italic>pol</jats:italic> is also derived from the <jats:italic>cam</jats:italic> mitotype with evolutionary modifications. Genes with known functions are conserved in all mitotypes, but clear variation in open reading frames (ORFs) with unknown functions among the six mitotypes was observed. Sequence relationship analysis showed that there has been genome compaction and inheritance in the course of <jats:italic>Brassica</jats:italic> mitotype evolution.</jats:p> </jats:sec> <jats:sec> <jats:title>Conclusions</jats:title> <jats:p>We have sequenced four <jats:italic>Brassica</jats:italic> mitotypes, compared six <jats:italic>Brassica</jats:italic> mitotypes and suggested a mechanism for mitochondrial genome formation in <jats:italic>Brassica</jats:italic>, including evolutionary events such as inheritance, duplication, rearrangement, genome compaction, and mutation.</jats:p> </jats:sec>