Comparison of Real-Time, Quantitative PCR with Molecular Beacons to Nested PCR and Culture Methods for Detection of<i>Mycobacterium avium</i>subsp.<i>paratuberculosis</i>in Bovine Fecal Samples

DOI Web Site 被引用文献1件
  • Ying Fang
    Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007
  • Wai-Hong Wu
    Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007
  • Jessica L. Pepper
    Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007
  • Jill L. Larsen
    Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007
  • Salvatore A. E. Marras
    Department of Molecular Genetics, Public Health Research Institute, New York, New York 10016
  • Eric. A. Nelson
    Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007
  • William B. Epperson
    Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007
  • Jane Christopher-Hennings
    Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007

書誌事項

公開日
2002-01
権利情報
  • https://journals.asm.org/non-commercial-tdm-license
DOI
  • 10.1128/jcm.40.1.287-291.2002
公開者
American Society for Microbiology

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説明

<jats:title>ABSTRACT</jats:title><jats:p>An automated PCR with fluorescent probes (molecular beacons) detected<jats:italic>Mycobacterium avium</jats:italic>subsp.<jats:italic>paratuberculosis</jats:italic>in bovine feces. When the PCR was compared with culture in testing 41 fecal samples, kappa scores of 0.94 to 0.96, a sensitivity of 93 to 96%, and a specificity of 92% were obtained. Results were quantitated by using a standard curve derived from a plasmid containing IS<jats:italic>900</jats:italic>. A minimum quantity of 1.7 × 10<jats:sup>−4</jats:sup>pg of DNA, correlating to 1 to 8 CFU, was detected.</jats:p>

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