Human inhibitory receptors Ig-like transcript 2 (ILT2) and ILT4 compete with CD8 for MHC class I binding and bind preferentially to HLA-G
-
- Mitsunori Shiroishi
- Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba-yama 07, Sendai 980-8579, Japan; Structural Biology Center, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, Japan; Department of Pathology and Immunology, Washington University School of Medicine, Box 8118, 660 South Euclid Avenue, St....
-
- Kouhei Tsumoto
- Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba-yama 07, Sendai 980-8579, Japan; Structural Biology Center, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, Japan; Department of Pathology and Immunology, Washington University School of Medicine, Box 8118, 660 South Euclid Avenue, St....
-
- Kimie Amano
- Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba-yama 07, Sendai 980-8579, Japan; Structural Biology Center, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, Japan; Department of Pathology and Immunology, Washington University School of Medicine, Box 8118, 660 South Euclid Avenue, St....
-
- Yasuo Shirakihara
- Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba-yama 07, Sendai 980-8579, Japan; Structural Biology Center, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, Japan; Department of Pathology and Immunology, Washington University School of Medicine, Box 8118, 660 South Euclid Avenue, St....
-
- Marco Colonna
- Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba-yama 07, Sendai 980-8579, Japan; Structural Biology Center, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, Japan; Department of Pathology and Immunology, Washington University School of Medicine, Box 8118, 660 South Euclid Avenue, St....
-
- Veronique M. Braud
- Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba-yama 07, Sendai 980-8579, Japan; Structural Biology Center, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, Japan; Department of Pathology and Immunology, Washington University School of Medicine, Box 8118, 660 South Euclid Avenue, St....
-
- David S. J. Allan
- Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba-yama 07, Sendai 980-8579, Japan; Structural Biology Center, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, Japan; Department of Pathology and Immunology, Washington University School of Medicine, Box 8118, 660 South Euclid Avenue, St....
-
- Azure Makadzange
- Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba-yama 07, Sendai 980-8579, Japan; Structural Biology Center, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, Japan; Department of Pathology and Immunology, Washington University School of Medicine, Box 8118, 660 South Euclid Avenue, St....
-
- Sarah Rowland-Jones
- Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba-yama 07, Sendai 980-8579, Japan; Structural Biology Center, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, Japan; Department of Pathology and Immunology, Washington University School of Medicine, Box 8118, 660 South Euclid Avenue, St....
-
- Benjamin Willcox
- Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba-yama 07, Sendai 980-8579, Japan; Structural Biology Center, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, Japan; Department of Pathology and Immunology, Washington University School of Medicine, Box 8118, 660 South Euclid Avenue, St....
-
- E. Yvonne Jones
- Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba-yama 07, Sendai 980-8579, Japan; Structural Biology Center, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, Japan; Department of Pathology and Immunology, Washington University School of Medicine, Box 8118, 660 South Euclid Avenue, St....
-
- P. Anton van der Merwe
- Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba-yama 07, Sendai 980-8579, Japan; Structural Biology Center, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, Japan; Department of Pathology and Immunology, Washington University School of Medicine, Box 8118, 660 South Euclid Avenue, St....
-
- Izumi Kumagai
- Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba-yama 07, Sendai 980-8579, Japan; Structural Biology Center, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, Japan; Department of Pathology and Immunology, Washington University School of Medicine, Box 8118, 660 South Euclid Avenue, St....
-
- Katsumi Maenaka
- Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba-yama 07, Sendai 980-8579, Japan; Structural Biology Center, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, Japan; Department of Pathology and Immunology, Washington University School of Medicine, Box 8118, 660 South Euclid Avenue, St....
Description
<jats:p>Ig-like transcript 4 (ILT4) (also known as leukocyte Ig-like receptor 2, CD85d, and LILRB2) is a cell surface receptor expressed mainly on myelomonocytic cells, whereas ILT2 (also known as leukocyte Ig-like receptor 1, CD85j, and LILRB1) is expressed on a wider range of immune cells including subsets of natural killer and T cells. Both ILTs contain immunoreceptor tyrosine-based inhibitory receptor motifs in their cytoplasmic tails that inhibit cellular responses by recruiting phosphatases such as SHP-1 (Src homology 2 domain containing tyrosine phosphatase 1). Although these ILTs have been shown to recognize a broad range of classical and nonclassical human MHC class I molecules (MHCIs), their precise binding properties remain controversial. We have used surface plasmon resonance to analyze the interaction of soluble forms of ILT4 and ILT2 with several MHCIs. Although the range of affinities measured was quite broad (<jats:italic>K</jats:italic><jats:sub>d</jats:sub>= 2–45 μM), some interesting differences were observed. ILT2 generally bound with a 2- to 3-fold higher affinity than ILT4 to the same MHCI. Furthermore, ILT2 and ILT4 bound to HLA-G with a 3- to 4-fold higher affinity than to classical MHCIs, suggesting that ILT/HLA-G recognition may play a dominant role in the regulation of natural killer, T, and myelomonocytic cell activation. Finally, we show that ILT2 and ILT4 effectively compete with CD8 for MHCI binding, raising the possibility that ILT2 modulates CD8<jats:sup>+</jats:sup>T cell activation by blocking the CD8 binding as well as by recruiting inhibitory molecules through its immunoreceptor tyrosine-based inhibitory receptor motif.</jats:p>
Journal
-
- Proceedings of the National Academy of Sciences
-
Proceedings of the National Academy of Sciences 100 (15), 8856-8861, 2003-07-09
Proceedings of the National Academy of Sciences
- Tweet
Keywords
- Models, Molecular
- MESH: Sequence Homology, Amino Acid
- Protein Conformation
- MESH: Membrane Glycoproteins
- MESH: Amino Acid Sequence
- MESH: Base Sequence
- CD8-Positive T-Lymphocytes
- MESH: Histocompatibility Antigens Class I
- MESH: Recombinant Proteins
- MESH: Protein Conformation
- Leukocyte Immunoglobulin-like Receptor B1
- HLA Antigens
- Receptors, Immunologic
- MESH: Antigens, CD
- MESH: HLA Antigens
- Membrane Glycoproteins
- MESH: Kinetics
- MESH: CD8-Positive T-Lymphocytes
- Recombinant Proteins
- MESH: Surface Plasmon Resonance
- Killer Cells, Natural
- [SDV.IMM]Life Sciences [q-bio]/Immunology
- MESH: Models, Molecular
- MESH: Killer Cells, Natural
- DNA, Complementary
- CD8 Antigens
- Molecular Sequence Data
- MESH: Binding, Competitive
- In Vitro Techniques
- Binding, Competitive
- Antigens, CD
- Humans
- Amino Acid Sequence
- MESH: Receptors, Immunologic
- HLA-G Antigens
- MESH: Humans
- MESH: Molecular Sequence Data
- Binding Sites
- Base Sequence
- Sequence Homology, Amino Acid
- Histocompatibility Antigens Class I
- MESH: DNA, Complementary
- Surface Plasmon Resonance
- Kinetics
- MESH: Binding Sites
- MESH: Antigens, CD8
Details 詳細情報について
-
- CRID
- 1360011145015926912
-
- NII Article ID
- 80016072694
-
- ISSN
- 10916490
- 00278424
-
- PubMed
- 12853576
-
- Data Source
-
- Crossref
- CiNii Articles
- OpenAIRE
