Nucleotide sequence of cloned cDNA coding for honeybee prepromelittin

DOI Web Site Web Site 被引用文献3件

書誌事項

公開日
1983-09
権利情報
  • http://onlinelibrary.wiley.com/termsAndConditions#vor
DOI
  • 10.1111/j.1432-1033.1983.tb07626.x
公開者
Wiley

この論文をさがす

説明

<jats:p>Total mRNA from venom glands of young queen bees was transcribed into cDNA and cloned into the <jats:italic>Pst</jats:italic>I site of plasmid pBR322. The nucleotide sequence of two clones with inserts containing genetic information for prepromelittin is presented. The longer insert encompasses 374 base pairs including 52 nucleotides before the initiation codon and a 3′ non‐coding region of 112 base pairs. The 70 amino acids of prepromelittin represent the total coding capacity of the mRNA from which this insert is derived. Southern blot analysis with this cloned cDNA has shown that it hybridizes with a single <jats:italic>Eco</jats:italic>RI fragment of honeybee DNA which contains about 3000 base pairs.</jats:p>

収録刊行物

被引用文献 (3)*注記

もっと見る

詳細情報 詳細情報について

問題の指摘

ページトップへ