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- Anat Biran
- Department of Molecular Cell Biology Weizmann Institute of Science Rehovot 76100 Israel
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- Lior Zada
- Department of Molecular Cell Biology Weizmann Institute of Science Rehovot 76100 Israel
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- Paula Abou Karam
- Department of Molecular Cell Biology Weizmann Institute of Science Rehovot 76100 Israel
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- Ezra Vadai
- Department of Molecular Cell Biology Weizmann Institute of Science Rehovot 76100 Israel
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- Lior Roitman
- Department of Molecular Cell Biology Weizmann Institute of Science Rehovot 76100 Israel
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- Yossi Ovadya
- Department of Molecular Cell Biology Weizmann Institute of Science Rehovot 76100 Israel
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- Ziv Porat
- Flow Cytometry Unit Biological Services Department Weizmann Institute of Science 76100 Rehovot Israel
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- Valery Krizhanovsky
- Department of Molecular Cell Biology Weizmann Institute of Science Rehovot 76100 Israel
抄録
<jats:title>Summary</jats:title><jats:p>Senescent cells are present in premalignant lesions and sites of tissue damage and accumulate in tissues with age. <jats:italic>In vivo</jats:italic> identification, quantification and characterization of senescent cells are challenging tasks that limit our understanding of the role of senescent cells in diseases and aging. Here, we present a new way to precisely quantify and identify senescent cells in tissues on a single‐cell basis. The method combines a senescence‐associated beta‐galactosidase assay with staining of molecular markers for cellular senescence and of cellular identity. By utilizing technology that combines flow cytometry with high‐content image analysis, we were able to quantify senescent cells in tumors, fibrotic tissues, and tissues of aged mice. Our approach also yielded the finding that senescent cells in tissues of aged mice are larger than nonsenescent cells. Thus, this method provides a basis for quantitative assessment of senescent cells and it offers proof of principle for combination of different markers of senescence. It paves the way for screening of senescent cells for identification of new senescence biomarkers, genes that bypass senescence or senolytic compounds that eliminate senescent cells, thus enabling a deeper understanding of the senescent state <jats:italic>in vivo</jats:italic>.</jats:p>
収録刊行物
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- Aging Cell
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Aging Cell 16 (4), 661-671, 2017-04-28
Wiley