Bovine endometrium responds differentially to age-matched short and long conceptuses†
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- José María Sánchez
- School of Agriculture and Food Science, University College Dublin, Belfield, Dublin, Ireland
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- Daniel J Mathew
- School of Agriculture and Food Science, University College Dublin, Belfield, Dublin, Ireland
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- Susanta K Behura
- Division of Animal Sciences, University of Missouri, Columbia, Missouri, USA
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- Claudia Passaro
- School of Agriculture and Food Science, University College Dublin, Belfield, Dublin, Ireland
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- Gilles Charpigny
- INRA, Biologie du Développement et Reproduction, Jouy en Josas, France
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- Stephen T Butler
- Animal and Grassland Research and Innovation Centre, Teagasc, Moorepark, Fermoy, Co. Cork, Ireland
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- Thomas E Spencer
- Division of Animal Sciences, University of Missouri, Columbia, Missouri, USA
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- Pat Lonergan
- School of Agriculture and Food Science, University College Dublin, Belfield, Dublin, Ireland
Description
<jats:title>Abstract</jats:title> <jats:p>This study combined in vitro production of bovine blastocysts, multiple embryo transfer techniques, and a conceptus-endometrial explant co-culture system to test the hypothesis that bovine endometrium exposed to long vs. short day 15 conceptuses would exhibit a different transcriptome profile reflective of potential for successful pregnancy establishment. Bovine endometrial explants collected at the late luteal stage of the estrous cycle were cultured in RPMI medium for 6 h with nothing (control), 100 ng/mL recombinant ovine interferon tau (IFNT), a long day 15 conceptus, or a short day 15 conceptus. Transcriptional profiling of the endometrial explants found that exposure of endometrium to IFNT, long conceptuses, or short conceptuses altered (P < 0.05) expression of 491, 498, and 230 transcripts, respectively, compared to the control. Further analysis revealed three categories of differentially expressed genes (DEG): (i) commonly responsive to exposure to IFNT and conceptuses, irrespective of size (n = 223); (ii) commonly responsive to IFNT and long conceptuses only (n = 168); and genes induced by the presence of a conceptus but independent of IFNT (n = 108). Of those 108 genes, 101 were exclusively induced by long conceptuses and functional analysis revealed that regulation of molecular function, magnesium-ion transmembrane transport, and clathrin coat assembly were the principal gene ontologies associated with these DEG. In conclusion, bovine endometrium responds differently to age-matched conceptuses of varying size in both an IFNT-dependent and -independent manner, which may be reflective of the likelihood of successful pregnancy establishment.</jats:p>
Journal
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- Biology of Reproduction
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Biology of Reproduction 101 (1), 26-39, 2019-04-12
Oxford University Press (OUP)
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Details 詳細情報について
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- CRID
- 1360011145267207680
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- ISSN
- 15297268
- 00063363
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- Data Source
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- Crossref