Ceramide-induced inhibition of T lymphocyte voltage-gated potassium channel is mediated by tyrosine kinases
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- Erich Gulbins
- Department of Physiology, University of Tübingen, Gmelinstrasse 5, 72076 Tübingen, Germany
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- Ildiko Szabo
- Department of Physiology, University of Tübingen, Gmelinstrasse 5, 72076 Tübingen, Germany
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- Katharina Baltzer
- Department of Physiology, University of Tübingen, Gmelinstrasse 5, 72076 Tübingen, Germany
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- Florian Lang
- Department of Physiology, University of Tübingen, Gmelinstrasse 5, 72076 Tübingen, Germany
抄録
<jats:p>The n-type K<jats:sup>+</jats:sup>channel (n-K<jats:sup>+</jats:sup>, K<jats:sub>v</jats:sub>1.3) in lymphocytes has been recently implicated in the regulation of Fas-induced programmed cell death. Here, we demonstrate that ceramide, a lipid metabolite synthesized upon Fas receptor ligation, inhibits n-K<jats:sup>+</jats:sup>channel activity and induces a tyrosine phosphorylation of the K<jats:sub>v</jats:sub>1.3 protein in Jurkat T lymphocytes. Tyrosine phosphorylation of the n-K<jats:sup>+</jats:sup>channel correlated with an activation of the Src-like tyrosine kinase p56lck upon cellular treatment with the ceramide analog C6-ceramide. Because genetic deficiency of p56lck or inhibition of Src-like tyrosine kinases by herbimycin A prevented ceramide-mediated n-K<jats:sup>+</jats:sup>channel inhibition and tyrosine phosphorylation, we propose a ceramide-initiated activation of p56lck resulting in tyrosine phosphorylation and inhibition of the n-K<jats:sup>+</jats:sup>channel protein.</jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 94 (14), 7661-7666, 1997-07-08
Proceedings of the National Academy of Sciences
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詳細情報 詳細情報について
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- CRID
- 1360011145283778048
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- ISSN
- 10916490
- 00278424
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- データソース種別
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- Crossref