Diesel Exhaust Particles in Lung Acutely Enhance Experimental Peripheral Thrombosis
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- Abderrahim Nemmar
- From the Laboratory of Pneumology, Unit of Lung Toxicology (A.N., P.H.M.H., B.N.), and Center for Molecular and Vascular Biology (J.V., M.F.H.), Kapholieke Universiteit Leuven, Leuven, Belgium, and Medical Research Council Toxicology Unit (D.D.), Leicester, United Kingdom.
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- Peter H.M. Hoet
- From the Laboratory of Pneumology, Unit of Lung Toxicology (A.N., P.H.M.H., B.N.), and Center for Molecular and Vascular Biology (J.V., M.F.H.), Kapholieke Universiteit Leuven, Leuven, Belgium, and Medical Research Council Toxicology Unit (D.D.), Leicester, United Kingdom.
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- David Dinsdale
- From the Laboratory of Pneumology, Unit of Lung Toxicology (A.N., P.H.M.H., B.N.), and Center for Molecular and Vascular Biology (J.V., M.F.H.), Kapholieke Universiteit Leuven, Leuven, Belgium, and Medical Research Council Toxicology Unit (D.D.), Leicester, United Kingdom.
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- Jozef Vermylen
- From the Laboratory of Pneumology, Unit of Lung Toxicology (A.N., P.H.M.H., B.N.), and Center for Molecular and Vascular Biology (J.V., M.F.H.), Kapholieke Universiteit Leuven, Leuven, Belgium, and Medical Research Council Toxicology Unit (D.D.), Leicester, United Kingdom.
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- Marc F. Hoylaerts
- From the Laboratory of Pneumology, Unit of Lung Toxicology (A.N., P.H.M.H., B.N.), and Center for Molecular and Vascular Biology (J.V., M.F.H.), Kapholieke Universiteit Leuven, Leuven, Belgium, and Medical Research Council Toxicology Unit (D.D.), Leicester, United Kingdom.
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- Benoit Nemery
- From the Laboratory of Pneumology, Unit of Lung Toxicology (A.N., P.H.M.H., B.N.), and Center for Molecular and Vascular Biology (J.V., M.F.H.), Kapholieke Universiteit Leuven, Leuven, Belgium, and Medical Research Council Toxicology Unit (D.D.), Leicester, United Kingdom.
Description
<jats:p> <jats:bold> <jats:italic>Background—</jats:italic> </jats:bold> Pollution by particulates has consistently been associated with increased cardiovascular morbidity and mortality, but a plausible biological basis for this association is lacking. </jats:p> <jats:p> <jats:bold> <jats:italic>Methods and Results—</jats:italic> </jats:bold> Diesel exhaust particles (DEPs) were instilled into the trachea of hamsters, and blood platelet activation, experimental thrombosis, and lung inflammation were studied. Doses of 5 to 500 μg of DEPs per animal induced neutrophil influx into the bronchoalveolar lavage fluid with elevation of protein and histamine but without lactate dehydrogenase release. The same doses enhanced experimental arterial and venous platelet rich-thrombus formation in vivo. Blood samples taken from hamsters 30 and 60 minutes after instillation of 50 μg of DEPs yielded accelerated aperture closure (ie, platelet activation) ex vivo, when analyzed in the Platelet Function Analyser (PFA-100). The direct addition of as little as 0.5 μg/mL DEPs to untreated hamster blood significantly shortened closure time in vitro. </jats:p> <jats:p> <jats:bold> <jats:italic>Conclusions—</jats:italic> </jats:bold> The intratracheal instillation of DEPs leads to lung inflammation as well as a rapid activation of circulating blood platelets. The kinetics of platelet activation are consistent with the reported clinical occurrence of thrombotic complications after exposure to pollutants. Our findings, therefore, provide a plausible explanation for the increase in cardiovascular morbidity and mortality accompanying urban air pollution. </jats:p>
Journal
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- Circulation
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Circulation 107 (8), 1202-1208, 2003-03-04
Ovid Technologies (Wolters Kluwer Health)
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Details 詳細情報について
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- CRID
- 1360011145304304128
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- NII Article ID
- 30022668271
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- ISSN
- 15244539
- 00097322
- http://id.crossref.org/issn/00097322
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- Data Source
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- Crossref
- CiNii Articles