Characterization of the katG gene encoding a catalase-peroxidase required for the isoniazid susceptibility of Mycobacterium tuberculosis
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- B Heym
- Unité de Génétique Moléculaire Bactérienne, Institut Pasteur, Paris, France.
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- Y Zhang
- Unité de Génétique Moléculaire Bactérienne, Institut Pasteur, Paris, France.
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- S Poulet
- Unité de Génétique Moléculaire Bactérienne, Institut Pasteur, Paris, France.
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- D Young
- Unité de Génétique Moléculaire Bactérienne, Institut Pasteur, Paris, France.
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- S T Cole
- Unité de Génétique Moléculaire Bactérienne, Institut Pasteur, Paris, France.
書誌事項
- 公開日
- 1993-07
- 権利情報
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- https://journals.asm.org/non-commercial-tdm-license
- DOI
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- 10.1128/jb.175.13.4255-4259.1993
- 公開者
- American Society for Microbiology
この論文をさがす
説明
<jats:p>The isoniazid susceptibility of Mycobacterium tuberculosis is mediated by the product of the katG gene which encodes the heme-containing enzyme catalase-peroxidase. In this study, the chromosomal location of katG has been established and its nucleotide sequence has been determined so that the primary structure of catalase-peroxidase could be predicted. The M. tuberculosis enzyme is an 80,000-dalton protein containing several motifs characteristic of peroxidases and shows strong similarity to other bacterial catalase-peroxidases. Expression of the katG gene in M. tuberculosis, M. smegmatis, and Escherichia coli was demonstrated by Western blotting (immunoblotting). Homologous genes were detected in other mycobacteria, even those which are naturally insensitive to isoniazid.</jats:p>
収録刊行物
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- Journal of Bacteriology
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Journal of Bacteriology 175 (13), 4255-4259, 1993-07
American Society for Microbiology