書誌事項
- 公開日
- 2009-10-25
- 権利情報
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- http://www.springer.com/tdm
- DOI
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- 10.1038/nchembio.259
- 公開者
- Springer Science and Business Media LLC
この論文をさがす
説明
We report a methodology for the ribosomal synthesis of backbone-cyclized peptides involving genetic code reprogramming to introduce one or more nonproteinogenic amino acids. Expression of linear peptides bearing a cysteine-proline dipeptide sequence followed by glycolic acid results in self-rearrangement to a C-terminal diketopiperadine-thioester, which non-enzymatically generates a cyclized peptide. We demonstrate the ribosomal synthesis of several naturally occurring backbone-cyclized peptides and a library based on a bicyclic scaffold, and we identify bioactive sequences by screening and deconvolution.
収録刊行物
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- Nature Chemical Biology
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Nature Chemical Biology 5 (12), 888-890, 2009-10-25
Springer Science and Business Media LLC
